Stanazolol derived ELISA as a sensitive forensic tool for the detection of multiple 17 alpha-methylated anabolics
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F20%3A43920335" target="_blank" >RIV/60461373:22330/20:43920335 - isvavai.cz</a>
Alternative codes found
RIV/60461373:22810/20:43920335
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0039128X19302405?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0039128X19302405?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.steroids.2019.108550" target="_blank" >10.1016/j.steroids.2019.108550</a>
Alternative languages
Result language
angličtina
Original language name
Stanazolol derived ELISA as a sensitive forensic tool for the detection of multiple 17 alpha-methylated anabolics
Original language description
Two valuable forensic tools based on enzyme-linked immunoassays (ELISAs) for the analysis of 17 alpha-methylated steroids were developed using haptens of stanazolol and its conjugates with biotin. Haptens containing terminal carboxylic group were conjugated to bovine serum albumin (BSA), rabbit serum albumin (RSA) or ovalburnin (OVA). Eight batches of antisera (RAbs) obtained by immunization of rabbits were tested in an indirect competitive ELISA system using immobilization of RSA conjugate (RSA/hapten) and competitor immobilization of the biotinylated conjugate (AB-ELISA) to avidin (avidin/hapten). The best results were achieved with the RAb 212 antibodies in RSA/ST-3 and avidin/ST-10 assembled variants. For the RSA/ST-3 system, an IC50 of 0.3 ng/mL and a detection limit of 0.02 ng/mL were measured. In case of avidin/ST-10 variant, IC50 was of 3.9 ng/mL and a detection limit of 0.57 ng/mL were obtained. The effect of solvent was tested as well as the stability of coated microtiter plates over four-month period. The cross-reactivity of the developed assays with other anabolic steroids was tested and high sensitivity towards 17 alpha-methylated steroids was observed. RSA/ST-3 assay showed significant cross-reactivity with 17 alpha-methyltestosterone (81.2%), oxymetholone (30.4%), methandienone (10.0%) and methyl dihydrotestosterone (7.7%). Similarly, in the avidin/ST-10 assay, 17 alpha-methyltestosterone (34.5%), mestanolone (32.1%), oxymetholone (22.7%), methandienone (14.2%), 9-dehydromethyltestosterone (12.5%) and oxandrolone (1.2%) exhibited high cross-reactivity. The functionality of the developed systems was verified by the successful identification of a series of 17 alpha-methylated anabolic steroids in a set of real samples including pharmaceutical preparations seized by the Police of the Czech Republic on the black market.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10609 - Biochemical research methods
Result continuities
Project
<a href="/en/project/VI20152020048" target="_blank" >VI20152020048: Development of New Methods and Tools for Detection and Investigation of Pharmacrime to Enhance Social Protection</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Steroids
ISSN
0039-128X
e-ISSN
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Volume of the periodical
155
Issue of the periodical within the volume
Březen 2020
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
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UT code for WoS article
000517349300001
EID of the result in the Scopus database
2-s2.0-85076856567