Tailor-made immunochromatographic test for the detection of multiple 17 alpha-methylated anabolics in dietary supplements

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F21%3A43923282" target="_blank" >RIV/60461373:22330/21:43923282 - isvavai.cz</a>

Result on the web

<a href="https://www.mdpi.com/2304-8158/10/4/741/htm" target="_blank" >https://www.mdpi.com/2304-8158/10/4/741/htm</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/foods10040741" target="_blank" >10.3390/foods10040741</a>

Result language

angličtina

Original language name

Tailor-made immunochromatographic test for the detection of multiple 17 alpha-methylated anabolics in dietary supplements

Original language description

The presented study describes the development of an immunostrip test for the detection of multiple 17 alpha-methylated AAS based on direct and indirect competitive principle using gold nanoparticles as a label. As a capture reagent on test lines conjugated stanazolol to rabbit serum albumin (RSA/ST-3) was used, the intensity of color formed in the test line of the AAS-positive sample was visually distinguishable from that of negative sample within 10 min. The optimized closed direct and indirect format of the test provided a similar visual detection limit (0.7 and 0.9 ng/mL, respectively). The most commonly orally abused AAS (17 alpha-methyltestosterone, methandienone, methyldihydrotestosterone, oxandrolone and oxymetholone) showed a strong cross-reaction. Developed immunostrips were successfully applied to analysis of artificially contaminated dietary supplements with 17 alpha-methylated AASs. The developed immunostrips offer potential as a useful user-friendly method for capturing suspicious dietary supplement samples with different contents of AAS at levels far below the usually used concentrations of AAS.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10406 - Analytical chemistry

Project

—

Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Foods

ISSN

2304-8158

e-ISSN

—

Volume of the periodical

10

Issue of the periodical within the volume

4

Country of publishing house

CH - SWITZERLAND

Number of pages

15

Pages from-to

1-15

UT code for WoS article

000643048600001

EID of the result in the Scopus database

2-s2.0-85104296720