Rapid Immunoassays for Detection of Anabolic Nortestosterone in Dietary Supplements

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F13%3A43896619" target="_blank" >RIV/60461373:22330/13:43896619 - isvavai.cz</a>
Result on the web
<a href="http://www.agriculturejournals.cz/publicFiles/100650.pdf" target="_blank" >http://www.agriculturejournals.cz/publicFiles/100650.pdf</a>
DOI - Digital Object Identifier
—

Result language
angličtina
Original language name
Rapid Immunoassays for Detection of Anabolic Nortestosterone in Dietary Supplements
Original language description
An enzyme immunoassay (ELISA) and an immunochromatographic strip were designed for a rapid detection of nortestosterone in dietary supplements. Two polyclonal antibodies and two types of nortestosterone-protein coating conjugates were tested to develop the most appropriate method. Under optimal experimental conditions, the most sensitive ELISA achieved the IC50 and the limit of detection values of 6.41 and 0.09 ng/mL, respectively. Assay specificity was tested measuring cross-reactivity of several steroids. The interference to the assay was negligible ({0.1%), expect for cross-reactivity with another frequently abused steroid testosterone (23%). The optimized gold particle-based immunochromatographic strip provided in semi-quantitative test a visual detection limit of 1 ng/mL. None of these methods shows the interferences using a filtrate from suspension of non-contaminated sample. After validation for particular matrices, the ELISA and the strip test could be useful tools for rapid an
Czech name
—
Czech description
—

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
—

Project
<a href="/en/project/VG20112015045" target="_blank" >VG20112015045: Rapid immunochemical tests for the detection of steroidal anabolics</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)