Development of colloidal carbon-based immunochromatographic strip for rapid detection of carbaryl in fruit juices.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F10%3A00024230" target="_blank" >RIV/60461373:22330/10:00024230 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Development of colloidal carbon-based immunochromatographic strip for rapid detection of carbaryl in fruit juices.

Original language description

A simple immunochromatographic assay for sensitive detection of insecticide carbaryl in fruit juices was developed. The test is based on inhibition format on a nitrocellulose membrane strip. The strip was separately coated with rabbit anti-swine antibody(control line) and carbaryl hapten-OVA conjugate (test line). Colour intensity on the test line was possible to recognize visually from that of negative sample within 10 min, with the detection limit of 5 ng mL-1. All characteristics of the visually evaluated strips were also measured quantitatively, and then the detection limit was 1.5 ng mL-1. Cross-reactions with other carbamate pesticides were not found (<1%). No matrix effects were observed when fruit juices (orange, apple, pear, banana) were diluted tenfold times before analyses. The results from immunochromatographical assay were in a good agreement with those obtained by enzyme-linked immunosorbent assay as reference method. All these facts indicate the potential of the immunoc

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/GP525%2F07%2FP273" target="_blank" >GP525/07/P273: The development of lateral flow immunoassay for detection of carbaryl in food</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

European Food Research and Technology

ISSN

1438-2377

e-ISSN

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Volume of the periodical

231

Issue of the periodical within the volume

3

Country of publishing house

DE - GERMANY

Number of pages

7

Pages from-to

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UT code for WoS article

000278737000013

EID of the result in the Scopus database

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