LC-MS/MS techniques for the analysis of steroid panel in human cerebrospinal fluid
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023761%3A_____%2F25%3AN0000014" target="_blank" >RIV/00023761:_____/25:N0000014 - isvavai.cz</a>
Alternative codes found
RIV/61383082:_____/25:00001576 RIV/00216208:11110/25:10504535 RIV/00216208:11130/25:10504535 RIV/00064203:_____/25:10504535
Result on the web
<a href="https://doi.org/10.1016/j.neuint.2025.106080" target="_blank" >https://doi.org/10.1016/j.neuint.2025.106080</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.neuint.2025.106080" target="_blank" >10.1016/j.neuint.2025.106080</a>
Alternative languages
Result language
angličtina
Original language name
LC-MS/MS techniques for the analysis of steroid panel in human cerebrospinal fluid
Original language description
The metabolic processes within the brain are reflected in the cerebrospinal fluid (CSF). It is in close contact with the nervous system, which is both target and source of multiple steroids. The aim of our study was to develop and validate robust, sensitive LC-MS/MS methods with and without derivatization step for the analysis of unconjugated steroids from all major steroid classes in CSF. The validation of the method without derivatization was performed for ten C19- steroids (dehydroepiandrosterone (DHEA), 7a-hydroxyDHEA, 7(3-hydroxyDHEA, 7ketoDHEA, testosterone, epitestosterone, dihydrotestosterone, 11-hydroxytestosterone, 11-ketotestosterone and androstenedione), ten C21- steroids (cortisol, 11-deoxycortisol, 21-deoxycortisol, cortisone, corticosterone, 11deoxycorticosterone, pregnenolone, progesterone, 17-hydroxyprogesterone, aldosterone) and three C18- steroids (estrone, estradiol, estriol). The method with derivatization is validated for determination of eleven C19steroids (testosterone, 11-ketodihydrotestosterone, 11-hydroxytestosterone, DHEA, 7a-hydroxyDHEA, 7(3hydroxyDHEA, 7-ketoDHEA, androstenedione, androsterone, epiandrosterone, 7(3-hydroxyepiandrosterone) and six C21- steroids (cortisol, cortisone, corticosterone, pregnenolone, 17-hydroxypregnenolone, progesterone) in CSF. The method without derivatization is applicable for the determination of the majority of steroids in CSF, except for pregnenolone, 17-hydroxypregnenolone and DHEA, for which the derivatization method provides better sensitivity. When analyzing CSF samples of normal pressure hydrocephalus (NPH) patients, 11-ketodihydrotestosterone, epitestosterone, androsterone, epiandrosterone, 7(3-hydroxyepiandrosterone, 7-ketoDHEA and 21-deoxycortisol were found to be below the LLOQ, suggesting that their presence is very limited. 17-hydroxypregnenolone, and 11-deoxycortisol were quantified for the first time, their CSF levels in NPH subjects are presented. We also observed significantly increased CSF levels of testosterone and 17-hydroxyprogesterone in men compared to women, both with NPH.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30202 - Endocrinology and metabolism (including diabetes, hormones)
Result continuities
Project
<a href="/en/project/NU23-04-00551" target="_blank" >NU23-04-00551: A Complex Multi-layer Diagnostic Battery for NPH</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Neurochemistry International
ISSN
0197-0186
e-ISSN
1872-9754
Volume of the periodical
191
Issue of the periodical within the volume
December
Country of publishing house
GB - UNITED KINGDOM
Number of pages
16
Pages from-to
106080
UT code for WoS article
001609422400001
EID of the result in the Scopus database
2-s2.0-105022182974