An LC-MS/MS method for the simultaneous quantification of 32 steroids in human plasma

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023761%3A_____%2F22%3AN0000010" target="_blank" >RIV/00023761:_____/22:N0000010 - isvavai.cz</a>

Alternative codes found

RIV/60461373:22330/22:43925435

Result on the web

<a href="https://www.sciencedirect.com/science/article/abs/pii/S1570023222001982" target="_blank" >https://www.sciencedirect.com/science/article/abs/pii/S1570023222001982</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jchromb.2022.123294" target="_blank" >10.1016/j.jchromb.2022.123294</a>

Result language

angličtina

Original language name

An LC-MS/MS method for the simultaneous quantification of 32 steroids in human plasma

Original language description

A development of robust and rapid method with simple sample preparation for the analysis of steroids of C18-, C19-, C21- families is of interest of many research groups. Here we present a novel LC-MS/MS method for the simultaneous quantification of 32 steroid hormones in human plasma. Twenty-two of them were analyzed directly without the need for derivatization, while ten were derivatized with 2-fluoro-1-methylpyridinium p-toluenesulfonate. The steroids were separated on a C18 column with a gradient elution consisting of methanol and water with the addition of 0.1% formic acid. The mass spectrometer was operated in positive ESI mode. Validation demonstrated that the method was applicable for the quantitative analysis of two C18- steroids (estrone, estradiol), nineteen C19- steroids (testosterone, epitestosterone, dihydrotestosterone, 11-ketodihydrotestosterone, 11 beta-hydroxyandrostenedione, 11 beta-hydroxytestosterone, 11-ketotestosterone, dehydroepiandrosterone, 7 alpha-hydroxydehydroepiandrosterone, 7 beta hydroxydehydroepiandrosterone, 7-ketodehydroepiandrosterone, androsterone, epiandrosterone, androstenedione, androstenediol, 5 alpha-androstane-3 alpha,17 beta-diol, 5 alpha-androstane-3 beta,17 beta-diol, 5 beta-androstane-3 alpha,17 beta-diol, 5 beta-androstane-3 beta,17 beta-diol), and eleven C21- steroids (cortisol, 21-deoxycortisol, 11-deoxycortisol, cortisone, corticosterone, 11-deoxycorticosterone, pregnenolone, 17 -hydroxypregnenolone, progesterone, 17-hydroxyprogesterone, 5 alpha-dihydroprogesterone). The lower limits of quantification are appropriate for analyses in both physiological and various pathophysiological conditions. The accuracy, intra-and inter-day precision values as well as stability tests were in accordance with FDA Guidelines. The method will be a useful tool in investigating the mechanisms of steroid-related diseases and will serve as a steppingstone for the development of other methods for steroid analyses in various biological matrices such as prostate tissue, cerebrospinal fluid, urine, seminal fluid, and saliva.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

<a href="/en/project/NU21J-01-00040" target="_blank" >NU21J-01-00040: Intraprostatic steroids: the role of alternative biosynthetic pathways and exposure to endocrine disruptors in the development of prostate cancer</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

ISSN

1570-0232

e-ISSN

1873-376X

Volume of the periodical

1201

Issue of the periodical within the volume

June

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

13

Pages from-to

Article Number123294

UT code for WoS article

000806812600002

EID of the result in the Scopus database

2-s2.0-85130593000