A rapid and effective RNA release procedure for virus detection in woody plants by reverse trancription-polymerase chain reaction

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F03%3A31" target="_blank" >RIV/00027006:_____/03:31 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

A rapid and effective RNA release procedure for virus detection in woody plants by reverse trancription-polymerase chain reaction

Original language description

A rapid and effective RNA release procedure (RNA-RP) for detection of the Apple stem pitting virus (ASPV) and the Apple stem grooving virus (ASGV) in woody plants by reverse transcription-polymerase chain reaction (RT-PCR) was developed. RNA-RP releasedRNA into crude homogenate. RNA-RP was compared with classical phenol/chlorophorm extraction of RNA. The RT-PCR detection of ASPV and ASGV was shown to be similar by both the RNA preparation procedures. RNA-RP in contrast to the classical phenol/chloroform procedure represents a reliable and easy-to band protocol convenient for routine virus detection. Leaf tissues, especially dormant bud leaves and leaves during blossom, were shown to be the most optimal material for detection of these viruses, irrespective of the used RNA preparation procedure.

Czech name

Rychlá a efektivní metoda RNA release pro detekci viru dřevin pomocí RT-PCR

Czech description

Rychlá a účinná metoda uvolnění RNA (RNA-RP) pro detekci ASPV a ASGV u jádrovin pomocí RT-PCR byla vyvinuta. RNA-RP byla porovnána s klasickou fenol-chloroformovou extrakcí RNA. Citlivost RT-PCR u obou metod extrakce byla podobná.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

<a href="/en/project/GA522%2F00%2FD025" target="_blank" >GA522/00/D025: Molecular and biological diagnosis of apple stem pitting virus (ASPV) in the Czech Republic</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2003

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Virologica

ISSN

0001-723X

e-ISSN

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Volume of the periodical

47

Issue of the periodical within the volume

3

Country of publishing house

SK - SLOVAKIA

Number of pages

5

Pages from-to

147-151

UT code for WoS article

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EID of the result in the Scopus database

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