Fluorescence-based AFLPs occur as the most suitable marker system for oiIseed rape cultivar identification

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F04%3A%23%23%23%23%23%2383" target="_blank" >RIV/00027006:_____/04:######83 - isvavai.cz</a>

Alternative codes found

RIV/00027006:_____/04:83

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Fluorescence-based AFLPs occur as the most suitable marker system for oiIseed rape cultivar identification

Original language description

Three different types of molecular markers, RAPD, SSR and fluorescence-based AFLP, were evaluated and compared for their ability to identify oilseed rape cultivars. The direct comparison of RAPD, SSR and AFLP approaches in cultivar identification showedlhal the AFLP methodology detected polymorphisms more efficiently than either RAPD or SSR methods. For the characterisation of six oilseed rape cultivars, 60 RAPD primers were tested and only eight of them (14%) detected sufficient levels of polymorphism.Five microsatellites out of fifteen tested were polymorphic, but in a111oci, except one, on1y two different alleles were detected. This resu1t indicated the limited degree of polymorphism found in Brassica napus. Each of the six tested AFLP combinationsdetected polymorphisms, the best combination (M-CAA/E-ACT) had 26% polymorphic peaks from a total of90 peaks and could distinguish the analysed cultivars and 4 out of 5 core lines of cultivars. The results presented show that florescence

Czech name

AFLP založené na fluorescenci je nejvhodnějším markerovacím systémem pro identifikaci odrůd řepky

Czech description

Three different types of molecular markers, RAPD, SSR and fluorescence-based AFLP, were evaluated and compared for their ability to identify oilseed rape cultivars. The direct comparison of RAPD, SSR and AFLP approaches in cultivar identification showedlhal the AFLP methodology detected polymorphisms more efficiently than either RAPD or SSR methods. For the characterisation of six oilseed rape cultivars, 60 RAPD primers were tested and only eight of them (14%) detected sufficient levels of polymorphism.Five microsatellites out of fifteen tested were polymorphic, but in a111oci, except one, on1y two different alleles were detected. This resu1t indicated the limited degree of polymorphism found in Brassica napus. Each of the six tested AFLP combinationsdetected polymorphisms, the best combination (M-CAA/E-ACT) had 26% polymorphic peaks from a total of90 peaks and could distinguish the analysed cultivars and 4 out of 5 core lines of cultivars. The results presented show that florescence

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GE - Plant cultivation

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Applied Genetics

ISSN

1234-1983

e-ISSN

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Volume of the periodical

45

Issue of the periodical within the volume

2

Country of publishing house

GB - UNITED KINGDOM

Number of pages

13

Pages from-to

161-173

UT code for WoS article

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EID of the result in the Scopus database

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