Ring test results of STS markers for leaf rust resistance genes in wheat

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F04%3A%23%23%23%23%23186" target="_blank" >RIV/00027006:_____/04:#####186 - isvavai.cz</a>

Alternative codes found

RIV/00027006:_____/04:186

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Ring test results of STS markers for leaf rust resistance genes in wheat

Original language description

DNA markers: STS, SCAR, CAPS and SSR, for 18 1eaf rust resistance genes in wheat are updated and primers for their amplification are listed. Five selected STS markers for leaf rust resistance genes Lr9, LrlO, Lr19, Lr24 and Lr28 were identified in the network of seven Euro- pean laboratories in 7 accessions, having the above mentioned genes. Near-isogenic lines of spring wheat Thatcher were used as positive controls. Markers for resistance genes Lr9, LrlO, Lr19, Lr24 were identified in all the seven laboratories as amplification products 1100 bp, 310 bp, 130 bp and 310 bp, respectively. The STS markers linked to resistance genes Lr9, LrlO, Lr19, Lr24 were found to be highly specific for these genes and will be useful in marker-assisted selection in wheat. However, amplification product 378 bp for resistance gene Lr28 was detected in all the accessions in all the seven laboratories, both in the accessions having the gene and those not having it. This marker needs to be improved.

Czech name

Výsledky kruhového testu STS markerů genové odolnosti rzi u pšenice

Czech description

DNA markers: STS, SCAR, CAPS and SSR, for 18 1eaf rust resistance genes in wheat are updated and primers for their amplification are listed. Five selected STS markers for leaf rust resistance genes Lr9, LrlO, Lr19, Lr24 and Lr28 were identified in the network of seven Euro- pean laboratories in 7 accessions, having the above mentioned genes. Near-isogenic lines of spring wheat Thatcher were used as positive controls. Markers for resistance genes Lr9, LrlO, Lr19, Lr24 were identified in all the seven laboratories as amplification products 1100 bp, 310 bp, 130 bp and 310 bp, respectively. The STS markers linked to resistance genes Lr9, LrlO, Lr19, Lr24 were found to be highly specific for these genes and will be useful in marker-assisted selection in wheat. However, amplification product 378 bp for resistance gene Lr28 was detected in all the accessions in all the seven laboratories, both in the accessions having the gene and those not having it. This marker needs to be improved.

Type

D - Article in proceedings

CEP classification

GE - Plant cultivation

OECD FORD branch

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Project

<a href="/en/project/QD1335" target="_blank" >QD1335: Development of new DNA marker types and evaluation of their potetial for barley genome investigation, marker assisted selection and genotype identification</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Microscopic fungi - host resistance genes, genetics and molecular research

ISBN

a

ISSN

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e-ISSN

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Number of pages

12

Pages from-to

87-98

Publisher name

Poznaň, Polsko

Place of publication

Poznaň, Posko

Event location

Poznaň, Polsko

Event date

Apr 4, 2003

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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