Verification of STS markers for leaf rust resistance genes of wheat by seven European laboratories
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F04%3A94" target="_blank" >RIV/00027006:_____/04:94 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Verification of STS markers for leaf rust resistance genes of wheat by seven European laboratories
Original language description
A set of Thatcher near-isogenic lines and two breeding lines were used to examine sequence tagged site (STS) markers linked to leaf rust resistance genes Lr9, LrlO, Lr19, Lr24, Lr28, Lr29, Lr35, and a simple sequenced repeat (SSR) marker for Lr39. The selected STS markers for resistance genes Lr9, LrlO, Lr19, Lr24 and Lr28 were identified in seven accessions by seven European laboratories. Near-isogenic lines of the spring wheat Thatcher were used as positive controls. Markers for resistance genes Lr9,LrlO, Lr19, Lr24 were identified in all seven laboratories as amplification products of 1100 bp, 310 bp, 130bp and 310 bp, respectively. The STS markers linked to resistance genes Lr9, LrlO, Lr19, Lr24, Lr29, Lr35 and the SSR marker for Lr39 were robustand highly specific for these genes and will be useful in marker-assisted selection in wheat. However, the amplification product of 378 bp that corresponded with resistance gene Lr28 was detected in all accessions including genotypes lack
Czech name
Ověření STS márkerů genů odolnosti proti rzi u pšenice sedmi evropskými laboratořemi
Czech description
Soubor téměř izogenních linií odvozených od odrůdy Tacher a dvě šlechtitelské linie byly použity pro prověření STS markérů spojených s odolností ke rzi pšeničné - geny Lr 9, Lr10, Lr19, Lr 24, Lr 28, Lr 29, Lr 35 a SSR markér pro Lr 39. STS markéry pro Lr 9, Lr10, Lr19, Lr 24, Lr 28 byly identifikovány v 7 liniích všemi participujícími laboratořemi. Markéry byly robustní a specifické. Pouze marker pro Lr 28 byl nespecifický a byl identifikován u všech analyzovaných linií. tento markér je třeba vylepšit.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/QC1336" target="_blank" >QC1336: Development and employment of new approaches for monitoring of genetic diversity and large-scale screening of genetic resources</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2004
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Cellular and Molecular Biology Letters
ISSN
1425-8153
e-ISSN
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Volume of the periodical
9
Issue of the periodical within the volume
4
Country of publishing house
PL - POLAND
Number of pages
310
Pages from-to
508-817
UT code for WoS article
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EID of the result in the Scopus database
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