Molecular basis of acetolactate synthase inhibitor resistance in Czech biotypes of kochia

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F04%3A%23%23%23%23%23788" target="_blank" >RIV/00027006:_____/04:#####788 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Molecular basis of acetolactate synthase inhibitor resistance in Czech biotypes of kochia

Original language description

Seven biotypes of kochia (Kochia scoparia (L.) Schrad.) resistant to chlorsulforon have been found in the Czech Republic since 1996. Several characteristics of these biotypes have been investigated so far. This is the first study on the molecular basis of their herbicide resistance to acetolactate synthase-inhibiting herbicides. Eight biotypes of kochia were examined for correspondence between restricion fragments of PCR amplification products and phenotypic resistance to chlorsurforon of individual plants. Two regions of the gene encoding acetolactate synthase (ALS) were amplified, digested with restriction enzymes and sequenced. The resistance to ALS-inhibiting herbicides in all Czech kochia biotypes studied was conferred by a mutation at codon 574 of the ALS gene. Analysis of the results of DNA sequencing indicated that the mutation induced a leucine for tryptophan substitution.

Czech name

Molekulární podstata rezistence českých biotypů bytele metlatého vůči inhibitorům acetolaktát syntázy

Czech description

Seven biotypes of kochia (Kochia scoparia (L.) Schrad.) resistant to chlorsulforon have been found in the Czech Republic since 1996. Several characteristics of these biotypes have been investigated so far. This is the first study on the molecular basis of their herbicide resistance to acetolactate synthase-inhibiting herbicides. Eight biotypes of kochia were examined for correspondence between restricion fragments of PCR amplification products and phenotypic resistance to chlorsurforon of individual plants. Two regions of the gene encoding acetolactate synthase (ALS) were amplified, digested with restriction enzymes and sequenced. The resistance to ALS-inhibiting herbicides in all Czech kochia biotypes studied was conferred by a mutation at codon 574 of the ALS gene. Analysis of the results of DNA sequencing indicated that the mutation induced a leucine for tryptophan substitution.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GA - Agricultural economics

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Zeitschrift für Pflanzenkrankheiten & Pflazenschutz

ISSN

0340-8159

e-ISSN

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Volume of the periodical

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Issue of the periodical within the volume

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Country of publishing house

DE - GERMANY

Number of pages

5

Pages from-to

515-519

UT code for WoS article

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EID of the result in the Scopus database

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