Molecular basis for the atrazine resistance in Czech kochia (Kochia scoparia (L.) Schrad.) biotypes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F04%3A%23%23%23%23%23814" target="_blank" >RIV/00027006:_____/04:#####814 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Molecular basis for the atrazine resistance in Czech kochia (Kochia scoparia (L.) Schrad.) biotypes

Original language description

The ploidy level and chromosome number were verified in nine Czech and two US populations of kochia (Kochia scoparia (L.) Schrad.) from various sites and with different susceptibility to acetolactate synthase (ALS)-inhibiting and atrazine herbicides. Radicles 5-15 mm in length from pre-germinated seeds of kochia were used for the karyological analysis. None out of eleven examined populations comprised a case where all 50 analyzed roots would have the diploid number of chromosomes (2n=18). A low frequency of tetraploidy (4n=36) was found in the population with multiple resistance to atrazine and ALS inhibitors. Besides diploids a high frequency of mixoplids was recorded. A region of the gene encoding D1 protein of photosystem II was amplified and sequenced in three Czech kochia biotypes to determine the molecular basis for the atrazine resistance. It was found out that the resistance in the "Bubny" and "Vršovice" kochia biotypes was conferred by a glycine for serine substitution at resi

Czech name

Molekulární podstata rezistence českých biotypů bytele metlatého (Kochia scoparia (L.) vůči atrazinu

Czech description

The ploidy level and chromosome number were verified in nine Czech and two US populations of kochia (Kochia scoparia (L.) Schrad.) from various sites and with different susceptibility to acetolactate synthase (ALS)-inhibiting and atrazine herbicides. Radicles 5-15 mm in length from pre-germinated seeds of kochia were used for the karyological analysis. None out of eleven examined populations comprised a case where all 50 analyzed roots would have the diploid number of chromosomes (2n=18). A low frequency of tetraploidy (4n=36) was found in the population with multiple resistance to atrazine and ALS inhibitors. Besides diploids a high frequency of mixoplids was recorded. A region of the gene encoding D1 protein of photosystem II was amplified and sequenced in three Czech kochia biotypes to determine the molecular basis for the atrazine resistance. It was found out that the resistance in the "Bubny" and "Vršovice" kochia biotypes was conferred by a glycine for serine substitution at resi

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GA - Agricultural economics

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Herbologia

ISSN

1840-0809

e-ISSN

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Volume of the periodical

5

Issue of the periodical within the volume

1

Country of publishing house

BA - BOSNIA AND HERZEGOVINA

Number of pages

12

Pages from-to

73-84

UT code for WoS article

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EID of the result in the Scopus database

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