Possibilities of Pyrenophora teres detection in barley leaf tissue

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F04%3A2423" target="_blank" >RIV/00027006:_____/04:2423 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Possibilities of Pyrenophora teres detection in barley leaf tissue

Original language description

Specific polymerase chain reaction (PCR) primers were developed from AFLP bands of DNA of Pyrenophora teres, the causal agent of net blotch on barley leaves. The primers were designed to specifically amplify DNA from P. teres f.sp. teres and allow its differentiation from P. teres f.sp. maculata, which is morphologically very similar to P. teres f.sp. teres in culture. The PCR amplification was carried out successfully from DNA extracted from fungi mycelium. The PCR assay was validated with 60 samples from 10 barley hosts originating from several regions across the Czech Republic. No cross reaction was observed with DNA of several other species like P. tritici repentis, P. graminea and Helminthosporium sativum. This method is prepared to be used to detect the pathogen from environmental samples for survey and management purposes.

Czech name

Možnosti detekce Pyrenophora teres v pletivech listů ječmene

Czech description

Specifická polymerázová řetězová reakce (PCR) byla vyvinuta na základě analýzy AFLP Pyrenophora teres, která způsobuje chorobu síťové listové skvrnitosti u ječmene. Primery byly navrženy tak, aby bylo možno specificky detekovat každou z forem Pyrenophorateres: Pyrenophora teres f. teres a Pyrenophora teres f. maculata. PCR reakce byly prováděny s DNA extrahovanou z mycelia. PCR byla validována amplifikací 60 vzorků z 10 odrůd ječmene uměle infikovaného oběma formami Pyrenophora teres. Žádná křížová reakce nebyla pozorována s druhy P. tritici-repentis, P. graminea a Helminthosporium sativum. Tato metoda je připravena pro detekci P. teres ve vzorcích a pro monitoring jejího výskytu.

Type

D - Article in proceedings

CEP classification

GF - Diseases, pests, weeds and plant protection

OECD FORD branch

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Project

<a href="/en/project/QC1361" target="_blank" >QC1361: Developent of a technique for the quantification of fungal pathogens based on Real-Time PCR approach.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Proceedings of 9-th International Barley Genetics Symposium

ISBN

80-902545-9-4

ISSN

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e-ISSN

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Number of pages

4

Pages from-to

705-708

Publisher name

Agricultural Research Institute Kromeriz

Place of publication

Brno

Event location

Brno

Event date

Jun 20, 2004

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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