Use of rooted leaves for screening of Brassica germplasm response to clubroot (Plasmodiophora brassicae) and downy mildew (Hyaloperonospora parasitica)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F05%3A%23%23%23%23%23495" target="_blank" >RIV/00027006:_____/05:#####495 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15310/05:00002490

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Use of rooted leaves for screening of Brassica germplasm response to clubroot (Plasmodiophora brassicae) and downy mildew (Hyaloperonospora parasitica)

Original language description

Rooted leaves and cotyledons of various cruciferous crops were used for the screening of Brassica germplasm response to two obligatory pathogens: clubroot (Plasmodiophora brassicae) and downy mildew (Hyaloperonospora parasitica). The development of rootswas induced after 20-hour dipping of leaf pedicels in the mixture of growth regulators: indolyl-butyric acid (10 mg/l) and nicotinic acid (5 mg/l). The detached rooted leaves and cotyledons were maintained in 250 ml plastic containers with perlite underfluorescent tubes in a growth chamber. With additional foliar fertilizing they remain vital for four months, producing clubroot galls on roots when dip-inoculated with Plasmodiophora spores and sporulating mycelia of downy mildew on leaves after drop inoculation with Hyaloperonospora parasitica. The possibilities of enhancing the sensitivity of this alternative assay in combination with immunochemical methods are discussed.

Czech name

Použití zakořeněných listů k průzkumu genofondu rodu Brassica na reakci vůči boulovitosti košťálovin (Plasmodiophora brassicae) a plísni zelné (Hyaloperonospora parasitica)

Czech description

Rooted leaves and cotyledons of various cruciferous crops were used for the screening of Brassica germplasm response to two obligatory pathogens: clubroot (Plasmodiophora brassicae) and downy mildew (Hyaloperonospora parasitica). The development of rootswas induced after 20-hour dipping of leaf pedicels in the mixture of growth regulators: indolyl-butyric acid (10 mg/l) and nicotinic acid (5 mg/l). The detached rooted leaves and cotyledons were maintained in 250 ml plastic containers with perlite underfluorescent tubes in a growth chamber. With additional foliar fertilizing they remain vital for four months, producing clubroot galls on roots when dip-inoculated with Plasmodiophora spores and sporulating mycelia of downy mildew on leaves after drop inoculation with Hyaloperonospora parasitica. The possibilities of enhancing the sensitivity of this alternative assay in combination with immunochemical methods are discussed.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GF - Diseases, pests, weeds and plant protection

OECD FORD branch

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Project

<a href="/en/project/QD1356" target="_blank" >QD1356: Development and utilization of effective methods for selection of genetic resources and production of Brassica vegetables initial breeding materials with desired traits</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Horticultural Science (Prague)

ISSN

0862-867X

e-ISSN

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Volume of the periodical

32

Issue of the periodical within the volume

1

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

5

Pages from-to

1-5

UT code for WoS article

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EID of the result in the Scopus database

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