Inhibitory specificity and insecticidal selectivity of alfa-amylase inhibitor from Phaseolus vulgaris

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F05%3A212" target="_blank" >RIV/00027006:_____/05:212 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Inhibitory specificity and insecticidal selectivity of alfa-amylase inhibitor from Phaseolus vulgaris

Original language description

The primary structure and proteolytic processing of the alfa-amylase isoinhibitor alfaAI-1 from common bean (Phaseolus vulgaris cv. Magna) was determined by protein chemistry techniques. The inhibitory specificity of alfaAI-1 was screened with a panel orthe diges- tive alfa-amylases from 30 species of insects, mites, gastropod, annelid worm, nematode and fungal phytopathogens with afrocus on agricultural pests and important model species. This in vitro analysis showed a selective inhibition of alfa-amylases from three orders or insect (Coleoptera, Hymenoptera and Diptera) and an inhibition of alfa-amylases of the annelid worm. The inhibitory potential of alfaAI-1 against several alfa-amylases was found to be modulated by pH. To understand how alfaAI-1discriminates among closely related alfa-amylases, the sequences of the alfa-amylases sensitive, respectively, insensitive to alfaAI-1 were compared, and the critical determinants were localized on the spatial alfa-amylase model. Based o

Czech name

Specifita alpha amylázového inhibitoru s Phaseolus vulgaris a jeho insekticidní a akaricidní aktivita.

Czech description

V této prácy byla řešena specifita alpha amylázového inhibitoru s Phaseolus vulgaris a jeho insekticidní a akaricidní aktivita.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GF - Diseases, pests, weeds and plant protection

OECD FORD branch

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Project

<a href="/en/project/GA522%2F04%2F1286" target="_blank" >GA522/04/1286: Insecticidal proteins targeted against insect midgut</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Phytochemistry

ISSN

0031-9422

e-ISSN

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Volume of the periodical

66

Issue of the periodical within the volume

1

Country of publishing house

US - UNITED STATES

Number of pages

9

Pages from-to

31-39

UT code for WoS article

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EID of the result in the Scopus database

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