Mechanism of Plum pox virus resistance in transgenic Prunus domestica L.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F06%3A1485" target="_blank" >RIV/00027006:_____/06:1485 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Mechanism of Plum pox virus resistance in transgenic Prunus domestica L.
Original language description
RNA silencing (posttranscriptional gene silencing-PTGS in plant) is a sequence-specific RNA degradation mechanism first discovered in transgenic plants functions as an adaptive immune system targeted against viruses. The hal1marks of RNA silencing, siRNAs (21 nt short and 27 nt long) were detected in transgenic plum, Prunus domestica clone C-5, resistant to Plum pox virus (PPV). The duplex siRNA (21-27-nt) were detected in non-inoculated healthy C5 plums. No such siRNAs were detected in healthy susceptible transgenic plum clone C-6 plums. When C6 plants were inoculated with PPV the 21-nt siRNA was clearly present. Similar short of21 nt was detected in a PPV infected C-5 (a single C5 plum was infected with PPV out of 30 PPV inoculated plants). However,PPV inoculated virus free clone C-5 showed the double banding pattern of siRNA (21-27-nt) as non-inoculated C5. The association of short (21 nt) and long (27nt) siRNA with PPV resistance is correspond with reports in herbaceous plant mode
Czech name
Mechanism of Plum pox virus resistance in transgenic Prunus domestica L.
Czech description
RNA silencing (posttranscriptional gene silencing-PTGS in plant) is a sequence-specific RNA degradation mechanism first discovered in transgenic plants functions as an adaptive immune system targeted against viruses. The hal1marks of RNA silencing, siRNAs (21 nt short and 27 nt long) were detected in transgenic plum, Prunus domestica clone C-5, resistant to Plum pox virus (PPV). The duplex siRNA (21-27-nt) were detected in non-inoculated healthy C5 plums. No such siRNAs were detected in healthy susceptible transgenic plum clone C-6 plums. When C6 plants were inoculated with PPV the 21-nt siRNA was clearly present. Similar short of21 nt was detected in a PPV infected C-5 (a single C5 plum was infected with PPV out of 30 PPV inoculated plants). However,PPV inoculated virus free clone C-5 showed the double banding pattern of siRNA (21-27-nt) as non-inoculated C5. The association of short (21 nt) and long (27nt) siRNA with PPV resistance is correspond with reports in herbaceous plant mode
Classification
Type
D - Article in proceedings
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2006
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Book of Proceedings of the "Eufrin Plum and Prune Working Group Meeting"
ISBN
80-902636-9-0
ISSN
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e-ISSN
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Number of pages
9
Pages from-to
61-69
Publisher name
Research and Breeding Institute of Pomology Holovousy Ltd.
Place of publication
Holovousy
Event location
Hradec Králové - Holovousy
Event date
Aug 31, 2006
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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