Behaviour of transgenic Plum pox virus-resistant Prunus domestica L. clone C5 grown in the open field under a high and permanent infection pressure of the PPV-REC strain

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F08%3A00000244" target="_blank" >RIV/00027006:_____/08:00000244 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Behaviour of transgenic Plum pox virus-resistant Prunus domestica L. clone C5 grown in the open field under a high and permanent infection pressure of the PPV-REC strain

Original language description

Prunus domestica L. clone C5 was inoculated with the recombinant strain (PPV-Rec) of the Plum pox virus (PPV). Non inoculated G5 plums were used as controls. Transgenic plums infected with PPV-Rec and control plants were evaluated for six years in the open field. Mild PPV symptoms, mild diffuse spots and rings, appeared on the basal leaves the second year after inoculation. The presence of PPV was confirmed by DAS-ELISA, ISEM, and RT-PCR. A reduction of symptoms and a decrease of the relative PPV concentration, as determined by DAS-ELISA in transgenic C5 plants, were observed from the third to the fifth year after virus inoculation. These results proved resitance of plum clone C5 to the PPV-Rec.

Czech name

Chování transgenní, k viru šarky švestky rezistentní Prunus domestica L., klon C5 v poli pod vysokým a permanentním infekčním tlakem kmenu PPV-Rec

Czech description

Prunus domestica L. klon C byla inokulována rekombinantním kmenem viru šarky švestky (PPV-Rec).Neinokulovanéé švestky C5 byly použity jeko kontrola. Transgenní švestky infikované PPV-Rec a kontrolní rostliny byly hodnoceny šest let v polních podmínkách.Mírné příznaky šarky se objevily druhý rok po inokulaci, přítomnost viru byla potvrzena ELISA, ISEM a RT-PCR. Příznaky i relativní koncentrace PPV od 3. do 5. roku dále slábly. Výsledky prokázaly rezistenci švestky klon C5 k PPV.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GF - Diseases, pests, weeds and plant protection

OECD FORD branch

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Project

<a href="/en/project/1B53054" target="_blank" >1B53054: The interaction of Plum pox, Prune dwarf and Apple chlorotic leafspot viruses with transgenic plants of Prunus domestica L., cv. Stanley, clone C-5 growed in experimental orchard.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Plant Pathology

ISSN

1125-4653

e-ISSN

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Volume of the periodical

90

Issue of the periodical within the volume

1 Suppl.

Country of publishing house

IT - ITALY

Number of pages

4

Pages from-to

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UT code for WoS article

000257453800007

EID of the result in the Scopus database

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