Interactions of Plum Pox Virus Strain Rec with Apple Chlorotic Leafspot Virus and Prune Dwarf Virus in Field-Grown Transgenic Plum Prunus domestica L., Clone C5

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F08%3A00000159" target="_blank" >RIV/00027006:_____/08:00000159 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Interactions of Plum Pox Virus Strain Rec with Apple Chlorotic Leafspot Virus and Prune Dwarf Virus in Field-Grown Transgenic Plum Prunus domestica L., Clone C5

Original language description

Transgenic plums, Prunus domestica L. clone C5, were inoculated by bud grafting with Plum pox virus (PPV-Rec, recombinant strain originated from plum), PPV-Rec + Apple chlorotic leafspot virus (ACLSVJ, PPV-Rec + Prune dwarf virus (PDV), and PPV-Rec + ACLSV + PDV. Non-inoculated transgenic plums served as controls. Plants were grown in an open field for 5 years. They were evaluated by visible symptoms, by DAS-ELISA and RT-PCR. Mild PPV symptoms, diffuse spots or rings appeared two years after inoculationin some leaves of plants artificially inoculated with PPV-Rec, PPV-Rec + ACLSV, PPV-Rec + PDV, and PPV-Rec + ACLSV + PDV. Severe PPV symptoms appeared in leaves of shoots growing from infected buds used for inoculation. During the following three years,further weakening of PPV symptoms was observed in transgenic plants. In 2007, very mild PPV symptoms were found in only a few leaves, and over 60%, resp. 70% of the C5 trees showed no PPV symptoms. ...

Czech name

Interakce viru šarky švestky, kmen PPV-Rec s viry chlorotické skvrnitosti jabloně a zakrslosti slivoně s transgenní švestkou Prunus domestica L., klon C5.

Czech description

Transgenní švestky Prunus domestica L., klon C5 byly inokulovány PPV-Rec a kombinacemi PPV-Rec + ACLSV, PPV-Rec+PDV a PPV-Rec + ACLSV + PDV. Jako kontrola byly neinokulované stromy transgenní švestky. Rostliny byly v polních podmínkách hodnoceny po dobupěti let stanovením příznaků, pomocí ELISA a RT-PCR. Byla zjištěna rezistence stromů k PPV i ke kombinovaným infekcím s dalšími viry.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GF - Diseases, pests, weeds and plant protection

OECD FORD branch

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Project

<a href="/en/project/1B53054" target="_blank" >1B53054: The interaction of Plum pox, Prune dwarf and Apple chlorotic leafspot viruses with transgenic plants of Prunus domestica L., cv. Stanley, clone C-5 growed in experimental orchard.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Protection Science

ISSN

1212-2580

e-ISSN

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Volume of the periodical

44

Issue of the periodical within the volume

1

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

5

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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