Rapid discrimination of the common species of the stored product pest Liposcelis(Psocoptera:Liposcelididae) from China and the Czech Republic, based on PCR-RFLP analysis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F08%3A00000161" target="_blank" >RIV/00027006:_____/08:00000161 - isvavai.cz</a>
Result on the web
—
DOI - Digital Object Identifier
—
Alternative languages
Result language
angličtina
Original language name
Rapid discrimination of the common species of the stored product pest Liposcelis(Psocoptera:Liposcelididae) from China and the Czech Republic, based on PCR-RFLP analysis
Original language description
Abstract. Psocids of the genus Liposcelis (Psocoptera: Liposcelididae) are stored product pests that are difficult to identify morphologically. A molecular method based on Restriction Fragment Length Polymorphism (RFLP) of the PCR-amplified 16S rDNA genewas developed for the rapid discrimination of four common species (L. bostrychophila, L. entomophila, L. decolor, and L. paeta). Different developmental stages and populations (P.R. China and Czech Republic) were tested. One DNA fragment of about 500 bpin length was amplified from genomic DNA and the fragment was then digested using the restriction endonuclease Dral. Identification of the relevant banding pattern allowed all the developmental stages and both sexes to be discriminated in the species tested.
Czech name
Rychlé rozlišení druhů skladištních škůdců rodu Liposcelis (Psocoptera:Liposcelididae) z Číny a České republiky na základě molekulární metody PCR-RFLP.
Czech description
Skladištní druhy pisivek rodu Liposcelis (Psocoptera: Liposcelididae) se obtížně určují na základě morfologických znaků. Proto byla vyvinuta spolehlivá molekulární metoda (RFLP, 16S rDNA) pro rychlé rozlišení čtyř druhů běžně se vyskytujících skladištních pisivek (L. bostrychophila, L. entomophila, L. decolor, L. paeta). Metoda byla testována na různých vývojových stadiích dvou geografických populací pisivek (Čína, Česká republika).
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GF - Diseases, pests, weeds and plant protection
OECD FORD branch
—
Result continuities
Project
<a href="/en/project/1P05ME733" target="_blank" >1P05ME733: Identification of stored product psocids - morphological studies on developmental stages of psocid species including comparisons of geographical strains from the Czech Republic and China</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2008
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
European Journal of Entomology
ISSN
1210-5759
e-ISSN
—
Volume of the periodical
105
Issue of the periodical within the volume
4
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
5
Pages from-to
—
UT code for WoS article
—
EID of the result in the Scopus database
—