Identification of European diplozoids (Diplozoidae, Monogenea) by restriction digestion of the ribosomal RNA internal transcribed spacer (ITS2)

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F03%3A00008781" target="_blank" >RIV/00216224:14310/03:00008781 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Identification of European diplozoids (Diplozoidae, Monogenea) by restriction digestion of the ribosomal RNA internal transcribed spacer (ITS2)
Original language description
The second internal transcribed spacer (ITS2) of ribosomal DNA of diplozoid parasites was amplified, sequenced and restriction enzyme digestion was performed to reveal restriction fragment length polymorphism (RFLP). Genetic markers for 8 species of European diplozoids were developed. As those diplozoids are very closely related, it was very difficult to find suitable enzymes to reveal different patterns for each species. Only a combination of digestion with AluI, HaeIII, HinfI, RsaI and SphI proved convenient to discriminate all species studied. Morphological analysis and measurements of the attachment apparatus of the studied species are also presented, together with comparison of the shape of trapeze spur and the anterior joining sclerites.
Czech name
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Czech description
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Publication year
2003
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
Bulletin of the Scandinavian Society for Parasitology, Proceedings of the XXI. Symposium of the Scandinavian Socity for Parasitology, Bergen, Norway 12-15 June, 2003
ISBN
0803-4907
ISSN
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e-ISSN
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Number of pages
2
Pages from-to
44-45
Publisher name
HS-Trykk A/S, Norway
Place of publication
Bergen, Norway
Event location
Bergen, Norway
Event date
Jan 1, 2003
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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