Protein chips applications
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F08%3A00000317" target="_blank" >RIV/00027006:_____/08:00000317 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Protein chips applications
Original language description
The aim of this work was the testing of a diagnostic tool for the detection of allergenic proteins from stored product mites/cockroaches and house dust mites. Species-specific antibodies were tested for specificity and cross-reactivity, and detection limit by ELISA method. In this work, protein chips were manufactured either manually, using a MicroCaster, or robotically, using a SpotArray. The proteins tested were six recombinant Kunitz-type protease inhibitors (PI), two of group A (with anti-cathepsinD and anti-trypsin activity), two of group B (with anti-trypsin and anti-chymotrypsin activity) and two of group C (with anti-cathepsin B activity), and the soybean Bowman-Birk inhibitor. Results of protease binding to each KPI and recognition of allergenic proteases showed the practicability of protein microarrays as a diagnostic tool for detection of mite/insect contaminants in foods and work environment.
Czech name
Aplikace proteinových čipů
Czech description
Cílem bylo testování diagnostických nástrojů pro detekci alergenních proteinů ze skladištních škůdců roztočů a švábů a prachových roztočů. Proteinové čipy se ukázaly jako vhodné a praktické detekční nástroje pro detekci roztočových a hmyzích kontaminantův potravinách a pracovním prostředí.
Classification
Type
C - Chapter in a specialist book
CEP classification
GM - Food industry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/1P04OC853.003" target="_blank" >1P04OC853.003: Screenig of digestive enzymes of mites as novel candidates for protein microarrays</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2008
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Book/collection name
Action COST 853. Agricultural biomarkers for array technology
ISBN
978-3-033-01770-2
Number of pages of the result
9
Pages from-to
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Number of pages of the book
104
Publisher name
Official Publications Office of European Community (OPOCE)
Place of publication
Bruxelles
UT code for WoS chapter
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