Feces Derived Allergens of Tyrophagus putrescentiae Reared on Dried Dog Food and Evidence of the Strong Nutritional Interaction between the Mite and Bacillus cereus Producing Protease Bacillolysins and Exo-chitinases

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F16%3A00003576" target="_blank" >RIV/00027006:_____/16:00003576 - isvavai.cz</a>

Alternative codes found

RIV/60460709:41210/16:70156 RIV/00216208:11310/16:10369113

Result on the web

<a href="http://dx.doi.org/10.3389/fphys.2016.00053" target="_blank" >http://dx.doi.org/10.3389/fphys.2016.00053</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fphys.2016.00053" target="_blank" >10.3389/fphys.2016.00053</a>

Result language

angličtina

Original language name

Feces Derived Allergens of Tyrophagus putrescentiae Reared on Dried Dog Food and Evidence of the Strong Nutritional Interaction between the Mite and Bacillus cereus Producing Protease Bacillolysins and Exo-chitinases

Original language description

The result identifies proteins in excrements of Tyrophagus putrescentiae and reveals interaction between the mite and Bacillus cereus. The 2D-E-MS/MS proteomic analysis identified mite trypsin allergen Tyr p 3, fatty acid-binding protein Tyr p 13 and putative mite allergens ferritin (Grp 30) and (poly)ubiquitins. Tyr p 3 was detected at different positions of the 2D-E; it indicates presence of zymogen at basic pI, and mature-enzyme form and enzyme fragment at acidic pI. The highest protease activity was assigned to trypsin-like proteases; lower activity was assigned to chymotrypsin-like proteases, and the cysteine protease cathepsin B-like had very low activity. Next, in the feces were identified exochitinases and proteases (bacillolysins) of B. cereus. The bacterial proteases are important toxins and exo-chitinases likely contribute to degradation of mite exuviae, mite bodies or food boluses consisting of chitin, including the peritrophic membrane. B. cereus was isolated and identified based on amplification and sequencing of 16S rRNA and motB genes.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

ED - Physiology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in Physiology

ISSN

1664-042X

e-ISSN

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Volume of the periodical

7

Issue of the periodical within the volume

February

Country of publishing house

US - UNITED STATES

Number of pages

14

Pages from-to

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UT code for WoS article

000370693200001

EID of the result in the Scopus database

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