Real-time PCR assay for the discrimination and quantification of wheat and barley strains of Wheat dwarf virus
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F12%3A00002041" target="_blank" >RIV/00027006:_____/12:00002041 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/s11262-011-0699-0" target="_blank" >http://dx.doi.org/10.1007/s11262-011-0699-0</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11262-011-0699-0" target="_blank" >10.1007/s11262-011-0699-0</a>
Alternative languages
Result language
angličtina
Original language name
Real-time PCR assay for the discrimination and quantification of wheat and barley strains of Wheat dwarf virus
Original language description
A rapid method for detection, discrimination and quantification of wheat and barley strains of wheat dwarf virus (WDV) was successfully developed. The sensitivity of quantification of the wheat and barley strains of WDV ranged from an average of 1.2 x107?1.2 x102 and from an average of 1.4 x 107?1.4 x104 copies of viral genome, respectively. These standard serial dilutions were applied to plant and vector tissues for virus titer calculations. Both strains of WDV were clearly discriminated by specific probes and melting curve analysis. Both TaqMan and SYBR Green technologies provided accurate and reliable methods for monitoring, detection, discrimination,and quantification of WDV.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GF - Diseases, pests, weeds and plant protection
OECD FORD branch
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Result continuities
Project
<a href="/en/project/ME10022" target="_blank" >ME10022: Ecology of cereal viruses and development of microarray chip and Real-time PCR platforms for identification and quantification of cereal viruses in natural and agricultural ecosystems.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Virus Genes
ISSN
0920-8569
e-ISSN
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Volume of the periodical
44
Issue of the periodical within the volume
2
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
7
Pages from-to
349-355
UT code for WoS article
000300905100027
EID of the result in the Scopus database
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