Evaluation of Reference Genes for the Relative Quantification of Apple stem groowing virus and Apple mosaic virus in Apple Trees
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F12%3A00002142" target="_blank" >RIV/00027006:_____/12:00002142 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/s13337-012-0065-4" target="_blank" >http://dx.doi.org/10.1007/s13337-012-0065-4</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s13337-012-0065-4" target="_blank" >10.1007/s13337-012-0065-4</a>
Alternative languages
Result language
angličtina
Original language name
Evaluation of Reference Genes for the Relative Quantification of Apple stem groowing virus and Apple mosaic virus in Apple Trees
Original language description
A SYBR Green-based one step RT-qPCR assay was developed for the detection and quantification of Apple stem grooving virus (ASGV) and Apple mosaic virus(ApMV). The RT-qPCR assay employed seven plant expressed genes?glyceraldehyde 3-phosphate dehydrogenase(GAPDH), 18S ribosomal RNA, ubiquitin, ribosomal protein S19, Rubisco, RNA polymerase subunit II and b-actin?as internal reference housekeeping genes in a relative quantification system in three apple cultivars. The average expression stability (M) found by GeNorm software suggest that GAPDH and S19 were the most stable reference genes. We propose employing GAPDH and S19 as housekeeping genes for accurate quantification of ASGV and ApMV in apple leaf samples. The detection limit for both viruses was found around 70 copies of viral genome by one-step RT-qPCR.
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GF - Diseases, pests, weeds and plant protection
OECD FORD branch
—
Result continuities
Project
—
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Indian Journal of Virology
ISSN
0970-2822
e-ISSN
—
Volume of the periodical
23
Issue of the periodical within the volume
1
Country of publishing house
IN - INDIA
Number of pages
3
Pages from-to
39-41
UT code for WoS article
000304157400007
EID of the result in the Scopus database
—