Validation of the 16S rDNA and COI DNA Barcoding Technique for Rapid Molecular Identification of Stored Product Psocids (Insecta: Psocodea: Liposcelididae)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F13%3A00002472" target="_blank" >RIV/00027006:_____/13:00002472 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1603/EC12163" target="_blank" >http://dx.doi.org/10.1603/EC12163</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1603/EC12163" target="_blank" >10.1603/EC12163</a>
Alternative languages
Result language
angličtina
Original language name
Validation of the 16S rDNA and COI DNA Barcoding Technique for Rapid Molecular Identification of Stored Product Psocids (Insecta: Psocodea: Liposcelididae)
Original language description
The goal of this study was to validate molecular identi?cation of stored-product psocids of genus Liposcelis based on 16S rDNA and cytochrome oxidase I (COI) DNA barcoding. Unidenti?ed liposcelids were compared with samples of seven common species (L. bostrychophila, L.brunnea, L. corrodens, L. decolor, L. entomophila, L. mendax, and L. paeta). The explored species liposcelids shared 98% sequence similarity for both the 16S rDNA and COI genes with the reference L. corrodens samples. These molecular results were veri?ed by morphological identi?cation of DK specimens, facilitated by SEM microphotography. TheDNAbarcoding method and the neighbor-joining phylogenetic analyses indicated that both the 16S rDNA and COI genes were suitable for Liposcelis species identifcation. DNA barcoding has great potential for use in fast and accurate liposcelid identification.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GF - Diseases, pests, weeds and plant protection
OECD FORD branch
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Result continuities
Project
<a href="/en/project/ME09080" target="_blank" >ME09080: Molecular diagnostic of some stored-product pests from China and Czech Republic as supplementation or compensation of conventional morphological identification</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Economic Entomology
ISSN
0022-0493
e-ISSN
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Volume of the periodical
106
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
419-425
UT code for WoS article
000314882200052
EID of the result in the Scopus database
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