Point mutations in the sodium channel gene conferring tau-fluvalinate resistance in Varroa destructor

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F14%3A00002891" target="_blank" >RIV/00027006:_____/14:00002891 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1002/ps.3679" target="_blank" >http://dx.doi.org/10.1002/ps.3679</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/ps.3679" target="_blank" >10.1002/ps.3679</a>

Result language
angličtina
Original language name
Point mutations in the sodium channel gene conferring tau-fluvalinate resistance in Varroa destructor
Original language description
Background: Sodium channels (SC) in mites and insects are target sites for pesticides including pyrethroids. Point mutations of SC gene were reported to change structural conformation of the protein and sensitivity to pesticides. To find mutations in SCgene of Varroa destructor (VmNa) we analyzed VmNa gene sequences available in GenBank and prepared specific primers for amplification of two fragments harboring the regions coding for (i) IIS4-IIS6 in positions 2837-3337 and (ii) IIIS4-3´terminus in positions 4737-6500 according to VmNa cDNA sequence AY259834. Results: Sensitive and resistant populations did not differ in amino acid sequences of the IIIS4-3´terminus VmNa region. The differences were found in the fragment IIS4-IIS6: In resistant population, mutation C(3004)>G resulted in substitution L(1002)> (codon ctg > gtg) in the position equivalent to L925 in house fly in the linker connecting segments IIS4 and IIS5. Additionally, mutation F(1052) >L (codon ttc > ctc) in the positio
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GJ - Diseases and animal vermin, veterinary medicine
OECD FORD branch
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Project
<a href="/en/project/QI111A119" target="_blank" >QI111A119: Identification and Causes of Prevention of Honeybee Colony Losses</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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