Detection of Moravian Isolates of GFLV: Comparison of Real-Time RT-PCR and ELISA

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F14%3A00003146" target="_blank" >RIV/00027006:_____/14:00003146 - isvavai.cz</a>
Alternative codes found
RIV/62156489:43510/14:00228515
Result on the web
<a href="http://dx.doi.org/10.3923/ijv.2014.263.271" target="_blank" >http://dx.doi.org/10.3923/ijv.2014.263.271</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3923/ijv.2014.263.271" target="_blank" >10.3923/ijv.2014.263.271</a>

Result language
angličtina
Original language name
Detection of Moravian Isolates of GFLV: Comparison of Real-Time RT-PCR and ELISA
Original language description
To detect Grapevine fanleaf virus (GFLV) in grapevine tissues, especially for certification of grapevine propagation materials, the Real-Time RT-PCR (Reverse Transcription - Polymerase Chain Reaction) TaqMan? assay was adopted and used. The comparison with DAS-ELISA detection method was made to verify higher sensitivity and reliability of Real-Time RT-PCR method. The Real-Time RT-PCR assay detected GFLV in all eight Moravian GFLV isolates tested, while DAS-ELISA was unable to detect GFLV in four of them.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GF - Diseases, pests, weeds and plant protection
OECD FORD branch
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Project
<a href="/en/project/QH91214" target="_blank" >QH91214: New biotechnolgical approaches for nepovirus resitance creation in grapevine rootstock cultivars.</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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