Emergence of quarantine Tobacco ringspot virus in Impatiens walleriana in the Czech Republic

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F15%3A00003253" target="_blank" >RIV/00027006:_____/15:00003253 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.17221/3/2015-PPS" target="_blank" >http://dx.doi.org/10.17221/3/2015-PPS</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17221/3/2015-PPS" target="_blank" >10.17221/3/2015-PPS</a>

Result language

angličtina

Original language name

Emergence of quarantine Tobacco ringspot virus in Impatiens walleriana in the Czech Republic

Original language description

Tobacco ringspot virus (TRSV) is a quarantine pathogen in Europe. During an official inspection in November 2011, Impatiens walleriana plants showing symptoms were found in a nursery in the Czech Republic. The causal agent of the disease was detected as Nepovirus group A by RT-PCR using specific primers of the Nepovirus group. Sequence analysis of PCR fragments confirms that the detected virus is TRSV. TRSV detection in these plants was further confirmed by ELISA and one-step RT-PCR using specific primers. The coat protein (CP) gene of the Czech TRSV isolate was sequenced, and the sequence analysis showed high identity of both nucleotide (99.28%) and amino acid (99.96%) levels compared with other known TRSV isolates from GenBank. Two amino acid motifs characteristic of nepoviruses, FDDY (FDAY) and FWGR (FYGR), were found equally at positions 80 and 497 of the TRSV CP genes, respectively, including the sequences described in this study.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GF - Diseases, pests, weeds and plant protection

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Protection Science

ISSN

1212-2580

e-ISSN

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Volume of the periodical

51

Issue of the periodical within the volume

3

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

8

Pages from-to

115-122

UT code for WoS article

000359020600001

EID of the result in the Scopus database

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