The partial molecular characterization of Plum pox virus infecting sweet cherry trees in the Czech Republic

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F08%3A00005554" target="_blank" >RIV/61989592:15310/08:00005554 - isvavai.cz</a>
Alternative codes found
RIV/25271121:_____/08:9P001959
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
The partial molecular characterization of Plum pox virus infecting sweet cherry trees in the Czech Republic
Original language description
The presence of PPV in sweet cherry (Prunus avium) has been previously reported within an experimental collection of cherry genotypes in Holovousy, Czech Republic. In this paper, the modified procedure of RNA isolation was successfully applied - one cycle of differential ultracentrifugation was inserted before standard RNA isolation using TRI Reagent. Five PPV virus isolates were detected by RT-PCR by commonly used P1/P2 primers. The strain specific PCR and sequence analysis of NIb-CP-3'NCR region (1375bp length) of PPV isolate 13/2 CZ identified that isolate as member of PPV-Rec group. Studies are in progress to confirm biological transmission of the isolate.
Czech name
Molekulární charakteristika viru šarky švestky infikující třešně v České Republice.
Czech description
Modifikovaný postup izolace RNA a RT-PCR pomocí primerů PPV1/2 byl úspěšně aplikován při detekci pěti izolátů PPV infikující třešně. Specifická RT-PCR a sekvenační analýza NIb-CP-3'NCR region (1375 bp length) PPV izolátu 13/2 CZ prokázala jeho příslušnost ke kmeni PPV-Rec.

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Publication year
2008
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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