Differentiation of Plum Pox Virus isolates by Single-Strand Conformation Polymorphism and Low-Stringency Single Specific Primer PCP analysis of HC-Pro genome region
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F09%3A00010038" target="_blank" >RIV/61989592:15310/09:00010038 - isvavai.cz</a>
Result on the web
—
DOI - Digital Object Identifier
—
Alternative languages
Result language
angličtina
Original language name
Differentiation of Plum Pox Virus isolates by Single-Strand Conformation Polymorphism and Low-Stringency Single Specific Primer PCP analysis of HC-Pro genome region
Original language description
Single-strand conformation polymorphism (SSCP) and low-stringency single specific primer (LSSP)-PCR were assessed for suitability and reliability in genotyping of Plum pox virus (PPV) isolates. Examined PPV isolates included 16 PPV-D, 12 PPV-M, and 14 PPV-Rec isolates collected in Czech Republic. The analysis was performed on the helper component protease (HC-Pro) region of the PPV genome. SSCP and LSSP-PCR allowed the differentiation of PPV strain, but SSCP was not able to distinguish isolates within the same strain. The individual genotyping of each PPV isolate was obtained by LSSP-PCR. Nevertheless, both SSCP and LSSP-PCR techniques are suitable for preliminary screening of genetic variability of plant RNA viruses.
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
—
Result continuities
Project
—
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Acta Virologica
ISSN
0001-723X
e-ISSN
—
Volume of the periodical
53
Issue of the periodical within the volume
1
Country of publishing house
SK - SLOVAKIA
Number of pages
4
Pages from-to
—
UT code for WoS article
—
EID of the result in the Scopus database
—