Detection of Clavibacter michiganensis subsp. Sepedonicus, the causal agent of potato ring rot, in the breeding and propagation materials of the three-stage control process

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F16%3A00003755" target="_blank" >RIV/00027006:_____/16:00003755 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Detection of Clavibacter michiganensis subsp. Sepedonicus, the causal agent of potato ring rot, in the breeding and propagation materials of the three-stage control process

Original language description

To maintain Clavibacter michiganensis subsp. sepedonicus-free (Cms-free) potato genetic resources, a three-stage process of checking limited lots of initial and new breeding potato materials was designed for small breeders in terms of costs, personnel and facilities. For an intensified evaluation of the presence of Cms in different vegetative stages within one growing season a mixture of 10 randomly selected artificially infected and healthy tubers of individual cultivars and breeding materials were prepared. In the first stage, a visual assessment of the vascular vessels of mother tubers was performed and at least 20% of each material was deemed positive, indicating that it should be discarded. In the second stage, one half of mother tubers were grown in the greenhouse and the other in a netting house. Plants in bloom and daughter tubers were evaluated. Within the second stage 1.4%–28% of each individual potato material could be excluded based on double-antibody sandwich enzyme-linked immunosorbent assays and SYBR Green real-time PCR assays, including a melting point analysis using PSA 1/R and CelA F/R primer sets and biological tests on aubergines. In the third stage, in vitro plants transferred from potato resources that tested negative in the previous stages were evaluated using the methods used in the second stage. The specificity of the antibodies and the two primer sets, PSA1/R and CelA F/R, were evaluated with 100% success.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GF - Diseases, pests, weeds and plant protection

OECD FORD branch

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Project

<a href="/en/project/QJ1310218" target="_blank" >QJ1310218: Reducing the risk of the occurrence of the causal agent of bacterial ring rot of potato in the breeding and propagation material.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Phytopathology and Pest Management

ISSN

2356-6507

e-ISSN

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Volume of the periodical

3

Issue of the periodical within the volume

2

Country of publishing house

EG - EGYPT

Number of pages

16

Pages from-to

48-63

UT code for WoS article

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EID of the result in the Scopus database

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