Wheat dwarf virus infectious clones allow to infect wheat and Triticum monococcum plants
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F19%3A00005099" target="_blank" >RIV/00027006:_____/19:00005099 - isvavai.cz</a>
Result on the web
<a href="https://www.agriculturejournals.cz/publicFiles/42_2018-PPS.pdf" target="_blank" >https://www.agriculturejournals.cz/publicFiles/42_2018-PPS.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17221/42/2018-PPS" target="_blank" >10.17221/42/2018-PPS</a>
Alternative languages
Result language
angličtina
Original language name
Wheat dwarf virus infectious clones allow to infect wheat and Triticum monococcum plants
Original language description
We constructed Wheat dwarf virus (WDV) infectious clones in the bacterial plasmids pUC18 and pIPKb002 and tested their ability to inoculate plants using Bio-Rad Helios Gene Gun biolistic inoculation method and Agrobacterium tumefaciens agroinoculation method, and we then compared them with the natural inoculation method via viruliferous P. alienus. Infected plants were generated using both infectious clones, whereas the agroinoculation method was able to produce strong systemic infection in all three tested cultivars of wheat and Triticum monococcum, comparable to plants inoculated by viruliferous P. alienus. Infection was confirmed by DAS-ELISA, and WDV titres were quantified using qPCR. The levels of remaining bacterial plasmid DNA were also confirmed to be zero.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plant Protection Science
ISSN
1212-2580
e-ISSN
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Volume of the periodical
55
Issue of the periodical within the volume
2
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
9
Pages from-to
81-89
UT code for WoS article
000458784900002
EID of the result in the Scopus database
2-s2.0-85063531283