Two mutations in the truncated Rep gene RBR domain delayed the Wheat dwarf virus infection in transgenic barley plants
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F18%3A00004852" target="_blank" >RIV/00027006:_____/18:00004852 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15310/18:73591181
Result on the web
<a href="http://dx.doi.org/10.1016/S2095-3119(18)62000-3" target="_blank" >http://dx.doi.org/10.1016/S2095-3119(18)62000-3</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/S2095-3119(18)62000-3" target="_blank" >10.1016/S2095-3119(18)62000-3</a>
Alternative languages
Result language
angličtina
Original language name
Two mutations in the truncated Rep gene RBR domain delayed the Wheat dwarf virus infection in transgenic barley plants
Original language description
Wheat dwarf virus (WDV), an important cereal pathogen, is closely related to Maize streak virus (MSV), a model virus of the Mastrevirus genus. Based on its similarity to known MSV resistance strategies, a truncated part of the WDV replication-associated (RepA) gene (WDVRepA215) and the WDV RepA gene with a mutated retinoblastoma-related protein (RBR) interaction domain (WDVRepA215RBRmut) were cloned into the pIPKb002 expression vector and transformed into immature embryos of spring barley cv. Golden Promise plants through Agrobacterium-mediated transformation. A detailed study of T1-generation plants infected by leafhoppers (Psammotettix alienus) fed on infection sources of variable strength was performed over a 5-week period encompassing the initial stages of virus infection. A DNA WDV TaqMan qPCR assay normalized using the DNA puroindoline-b SYBR Green qPCR assay for samples on a per week basis revealed an approximately 2-week delay in WDVRepA215RBR(mut) plants to WDVRepA215 plants before significant increases in the WDV viral levels occurred. Both WDVRepA215 and WDVRepA215RBRmut plants showed similar levels of transgenic transcripts over the screened period; however, the transgenic plants also showed increased numbers of infected plants compared to the control plants.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)
Result continuities
Project
<a href="/en/project/LH12161" target="_blank" >LH12161: Functional genomics study of Wheat dwarf virus for the identification of source of resistance and characterization of pathogenicity determinates</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Integrative Agriculture
ISSN
2095-3119
e-ISSN
—
Volume of the periodical
17
Issue of the periodical within the volume
11
Country of publishing house
CN - CHINA
Number of pages
9
Pages from-to
2492-2500
UT code for WoS article
000450126000013
EID of the result in the Scopus database
2-s2.0-85056398519