Carlina oxide from Carlina acaulis root essential oil acts as a potent mosquito larvicide
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F19%3A00005388" target="_blank" >RIV/00027006:_____/19:00005388 - isvavai.cz</a>
Alternative codes found
RIV/60460709:41210/19:80923
Result on the web
<a href="https://www.sciencedirect.com/science/article/abs/pii/S0926669019303711" target="_blank" >https://www.sciencedirect.com/science/article/abs/pii/S0926669019303711</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.indcrop.2019.05.037" target="_blank" >10.1016/j.indcrop.2019.05.037</a>
Alternative languages
Result language
angličtina
Original language name
Carlina oxide from Carlina acaulis root essential oil acts as a potent mosquito larvicide
Original language description
Developing effective larvicides for mosquito control is being challenging due to the quick development of resistance in targeted vectors. Botanical products can help, due to their multiple mechanisms of action coupled with eco-friendly features. Carlina acaulis (Asteraceae) is an Alpine perennial herb used as a traditional remedy and food. Its root essential oil (EO) has antimicrobial and antitrypanosomal properties, and is currently listed among botanicals employable in food supplements. Its insecticidal activity has not been explored so far. Here, after analysing the C. acaulis EO chemical composition by GC-MS and NMR, we detected extremely high larvicidal activity of this EO and its main constituent, carlina oxide, against Culex quinquefasciatus larvae by using the standard WHO protocol. LC50 were 1.31 and 1.39 mu g mL(-1), respectively. 24-h exposure to both products triggered significant mortality rates for five days post-treatment. Larvicidal tests on a wider scale confirmed > 95% larvicidal effectiveness of the EO and carlina oxide tested at 1.25 mu g L-1. Their non-target impact was evaluated through experiments on Daphnia magna adults. Both showed significantly lower toxicity if compared to cypermethrin. To shed light on the modes of action, carlina oxide was tested for anti-acetylcholinesterase activity by the Ellman method, with lower performances over galantamine. A moderate antioxidant potential was observed using DPPH and ABTS assays, since it has a role for preserving the shelf-life of herbal-based insecticides. Finally, cytotoxicity on vertebrate cells was noted, testing carlina oxide on human dermis, HCT116 and MDA-MB231 cell lines by MTT assay. Overall, the outstanding toxicity of the tested products make them excellent candidates to develop novel mosquito larvicides for real-world applications.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Industrial Crops and Products
ISSN
0926-6690
e-ISSN
1872-633X
Volume of the periodical
137
Issue of the periodical within the volume
OCT 1 2019
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
11
Pages from-to
356-366
UT code for WoS article
000473376800044
EID of the result in the Scopus database
2-s2.0-85065894236