Detecting soybean and milk in dairy and soy products with post-PCR high resolution melting assays

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F20%3A10145114" target="_blank" >RIV/00027006:_____/20:10145114 - isvavai.cz</a>

Result on the web

<a href="https://www.agriculturejournals.cz/publicFiles/125_2020-CJFS.pdf" target="_blank" >https://www.agriculturejournals.cz/publicFiles/125_2020-CJFS.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17221/125/2020-CJFS" target="_blank" >10.17221/125/2020-CJFS</a>

Result language

angličtina

Original language name

Detecting soybean and milk in dairy and soy products with post-PCR high resolution melting assays

Original language description

We have developed and validated post-PCR (polymerase chain reaction) high resolution melting (HRM) based assays that allow identifying the presence of two major allergenic foods - soybean and bovine milk - in food products. A new set of primers for PCR was developed for detection of the gene encoding the soybean protein lectin. The assay was validated using reference samples and used for analysis of artificially prepared mixed samples of dairy and soy products of different matrices, as well as of real products available in the market (spray creams). The limits of detection (LODs) of the soybean (8 copies) and bovine milk (4 copies) assays were lower compared to LODs of other previously published PCR-based assays. The analysis of several commercial spray creams revealed an undeclared presence of soybean in one of the samples. The newly developed HRM assays are precise and robust alternatives for the control of food composition and falsification by competent authorities.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40401 - Agricultural biotechnology and food biotechnology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

CZECH JOURNAL OF FOOD SCIENCES

ISSN

1212-1800

e-ISSN

—

Volume of the periodical

38

Issue of the periodical within the volume

4

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

6

Pages from-to

209-214

UT code for WoS article

000564318800002

EID of the result in the Scopus database

2-s2.0-85094324025