Primers and visualization of LAMP: A rapid molecular identification method for Liposcelis entomophila (Enderlein) (Psocodea: Liposcelididae)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F21%3A10174583" target="_blank" >RIV/00027006:_____/21:10174583 - isvavai.cz</a>
Alternative codes found
RIV/60460709:41210/21:N0000120
Result on the web
<a href="https://www.sciencedirect.com/journal/journal-of-stored-products-research/vol/93/suppl/C" target="_blank" >https://www.sciencedirect.com/journal/journal-of-stored-products-research/vol/93/suppl/C</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jspr.2021.101855" target="_blank" >10.1016/j.jspr.2021.101855</a>
Alternative languages
Result language
angličtina
Original language name
Primers and visualization of LAMP: A rapid molecular identification method for Liposcelis entomophila (Enderlein) (Psocodea: Liposcelididae)
Original language description
Liposcelis entomophila (Psocodea: Liposcelididae) is one of the most economically important booklice in the world. This species damages stored grain and represents an allergenic health risk to humans. Because of the small size of booklice, they are difficult to identify using morphological characteristics. Traditional molecular identification, such as DNA barcoding and qRT-PCR, requires specialized equipment and takes a long time, which is not appropriate for practical usage by plant health inspectors at ports. Therefore, for storage psocid identification, we suggested a simple, rapid and relatively new technique known as loop-mediated isothermal amplification (LAMP). In our study, a new rapid molecular identification method using the LAMP technique based on the mitochondrial gene cytochrome c oxidase I (COI) was developed for L. entomophila with high specificity and efficiency. Evident amplification results could be presented if the DNA concentration was 0.001 ng/mu L. Visual detection was also possible with our LAMP primers using hydroxy naphthol blue (HNB), relying on the clear change in color from violet to sky blue after amplification. This method successfully determined unknown species that were newly collected from grain stores and may facilitate L. entomophila identification in plant quarantine and stored product protection.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)
Result continuities
Project
<a href="/en/project/LTACH19029" target="_blank" >LTACH19029: The invasive mechanisms of economically important stored insect pests endangering Chinese and European international trades and phytosanitary technical systems for their managment</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
JOURNAL OF STORED PRODUCTS RESEARCH
ISSN
0022-474X
e-ISSN
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Volume of the periodical
93
Issue of the periodical within the volume
SEP 2021
Country of publishing house
GB - UNITED KINGDOM
Number of pages
7
Pages from-to
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UT code for WoS article
000686895900009
EID of the result in the Scopus database
2-s2.0-85111786922