Comparison of methods to extract PCR-amplifi able DNA from fruit, herbal and black teas

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F21%3A10174604" target="_blank" >RIV/00027006:_____/21:10174604 - isvavai.cz</a>
Alternative codes found
RIV/60461373:22330/21:43923686
Result on the web
<a href="https://www.agriculturejournals.cz/publicFiles/24_2021-CJFS.pdf" target="_blank" >https://www.agriculturejournals.cz/publicFiles/24_2021-CJFS.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17221/24/2021-CJFS" target="_blank" >10.17221/24/2021-CJFS</a>

Result language
angličtina
Original language name
Comparison of methods to extract PCR-amplifi able DNA from fruit, herbal and black teas
Original language description
The success of polymerase chain reaction (PCR) assay depends on template deoxyribonucleic acid (DNA) being sufficient with respect to both quantity and quality. Some biological materials contain compounds which inhibit the functioning of DNA polymerase and thus need to be removed as part of the DNA extraction procedure. The aim of the present experiments was to optimise the process of DNA isolation from various types of black, fruit and herbal teas. A comparison was made between two cetyltrimethylammonium bromide (CTAB)-based protocols and two commercially available DNA purification kits. The yield and integrity of the extracted DNA were monitored both spectrophotometrically and using agarose gel electrophoresis. The presence/absence of inhibitors in the DNA preparations was checked by running quantitative real-time PCRs. The optimal protocol was deemed to be the CTAB method described in ISO 21571:2005, so this method is recommended for the routine sample analysis of tea products.
Czech name
—
Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Project
<a href="/en/project/QJ1530272" target="_blank" >QJ1530272: Complex strategies for effective detection of food fraud in the chain production-consumer</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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