System for detection of GM potatoes and wheat in food - sborník

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F03%3A00008841" target="_blank" >RIV/60461373:22330/03:00008841 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
System for detection of GM potatoes and wheat in food - sborník
Original language description
The detection of genetically modified organisms is often pursued by the polymerase chain reaction (PCR). The detection includes basically three steps: DNA extraction from the sample, PCR with detection of products, and data evaluation. In the presented report a suitable protocol for the DNA isolation was searched for. From the published methods, five were chosen and used for the isolation of the DNA from wheat, soybean, potatoes and corn. The methods were evaluated by means of different parameters, likethe DNA purity, amplifiability by PCR, cost and duration of the procedure. The modified CTAB method and DNeasyTM Plant Mini Kit were found the most suitable methods of extraction. Then the selection of optimal conditions for detection of transgenic wheat and transgenic potato was performed. The samples were tested for possible occurrence of sequences of transgenic DNA that are used in commonly available genetically modified products. The most frequent sequences, regulating expression of
Czech name
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Czech description
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Publication year
2003
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
First International Symposium on Recent Advances in Food Analysis
ISBN
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ISSN
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e-ISSN
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Number of pages
1
Pages from-to
128
Publisher name
FIRMA -JK
Place of publication
Praha
Event location
Praha
Event date
Nov 5, 2003
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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