Morphological, Cytological, and Molecular Comparison between Diploid and Induced Autotetraploids of Callisia fragrans (Lindl.) Woodson

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F22%3A10175799" target="_blank" >RIV/00027006:_____/22:10175799 - isvavai.cz</a>

Alternative codes found

RIV/60460709:41210/22:91508 RIV/60460709:41340/22:91508

Result on the web

<a href="https://www.mdpi.com/2073-4395/12/10/2520/pdf?version=1666143704" target="_blank" >https://www.mdpi.com/2073-4395/12/10/2520/pdf?version=1666143704</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/agronomy12102520" target="_blank" >10.3390/agronomy12102520</a>

Result language

angličtina

Original language name

Morphological, Cytological, and Molecular Comparison between Diploid and Induced Autotetraploids of Callisia fragrans (Lindl.) Woodson

Original language description

The objective of the current study was to assess the efficiency of oryzalin in inducing polyploids in Callisia fragrans (Lindl.) Woodson by in vitro polyploidization. Shoot tips were subjected to Murashige and Skoog (MS) medium containing oryzalin at concentrations 1, 5, and 10 microM for 4 and 8 weeks. Further, the ploidy levels of the plants were confirmed using flow cytometry and chromosome counting. Among all treatments, six tetraploid plants (2n = 4x = 24) were obtained after 8 weeks in MS medium containing 5 microM oryzalin. Upon ex vitro transfer, tetraploid plants were morphologically distinct compared to diploid plants. The size of the leaf and flower increased significantly and nearly doubled when compared to the mother diploid plant. Further, inductively coupled plasma-optical emission spectrometry showed that tetraploid plants exhibited significantly higher sodium, iron, and calcium content, and the potassium content was increased by 100%. Molecular analysis utilizing iPBS and CDDP markers was tested for the first time in C. fragrans to assess the variation between tetraploid and diploid genotypes. Both the markers generated three major clusters, indicating a clear distinction between diploid, tetraploid, and the mixoploid genotypes. In conclusion, in vitro polyploidization using oryzalin could effectively induce polyploids in this and related species. Additionally, the results obtained in this study will provide a basis for future breeding opportunities in this species.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Agronomy-Basel

ISSN

2073-4395

e-ISSN

—

Volume of the periodical

12

Issue of the periodical within the volume

10

Country of publishing house

CH - SWITZERLAND

Number of pages

16

Pages from-to

2520

UT code for WoS article

000872038700001

EID of the result in the Scopus database

2-s2.0-85140461175