In Vitro Polyploidization of Thymus vulgaris L. and Its Effect on Composition of Essential Oils

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F21%3A43903675" target="_blank" >RIV/60076658:12310/21:43903675 - isvavai.cz</a>

Alternative codes found

RIV/86652079:_____/21:00542600

Result on the web

<a href="https://www.mdpi.com/2073-4395/11/3/596" target="_blank" >https://www.mdpi.com/2073-4395/11/3/596</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/agronomy11030596" target="_blank" >10.3390/agronomy11030596</a>

Result language

angličtina

Original language name

In Vitro Polyploidization of Thymus vulgaris L. and Its Effect on Composition of Essential Oils

Original language description

The aim of this work was to find an effective protocol for in vitro propagation and to perform the in vitro polyploidization of diploid Thymus vulgaris (2n = 30) using two experimental methods based on the use of oryzalin, an antimitotic agent. The ploidy level of the obtained shoots was checked by flow cytometric analysis. The most efficient conditions for inducing polyploidy were oryzalin concentrations of 0.346 and 1.73 mg L-1 present in the medium for two weeks. The vital polyploid shoots were multiplied for further evaluation, rooting and final transfer to nonsterile glasshouse and field conditions. The chemical compositions of the essential oils (EOs)-which were obtained from dried field grown plants by steam distillation-were analyzed by gas chromatography/mass spectrometry (GC/MS). The identified substances contributed approximately 95% to the total peak area. Statistical analysis revealed that the tetraploid subclone and the diploid reference plant do not differ in total terpene content, but they do differ in the relative proportions of all the individual terpenes with the exception of alpha-pinene and UN5, indicating that both clones produce EOs of different quality. The obtained results showed the possibility of developing more efficient botanical insecticides based on EOs obtained from the tetraploid plants.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Project

<a href="/en/project/LO1415" target="_blank" >LO1415: CzechGlobe 2020 – Development of the Centre of Global Climate Change Impacts Studies</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Agronomy

ISSN

2073-4395

e-ISSN

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Volume of the periodical

11

Issue of the periodical within the volume

3

Country of publishing house

CH - SWITZERLAND

Number of pages

11

Pages from-to

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UT code for WoS article

000633192200001

EID of the result in the Scopus database

2-s2.0-85108842407