Molecular characterization of watermelon mosaic virus isolates infecting zucchini and pumpkin plants in the Czech Republic

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F22%3A10175800" target="_blank" >RIV/00027006:_____/22:10175800 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Molecular characterization of watermelon mosaic virus isolates infecting zucchini and pumpkin plants in the Czech Republic

Original language description

Watermelon mosaic virus (WMV) is among the most important potyviruses infecting cucurbit vegetables in the Czech Republic. The occurrence of WMV in the eastern part of the Czech Republic was first reported by Svoboda (2011) based on ELISA testing. In 2019, symptoms of mosaic, leaf deformations, and fruit discoloration occurred on pumpkin and zucchini plants in a private garden in the western part of the Czech Republic, where WMV had not been reported before. High throughput sequencing was used for further characterization of the virus. The full WMV genome (acc. n MW188031) consists of 10,027 nt. One statistically significant recombination event was detected using the RDP4 program; the detected event was positive for six out of seven algorithms implemented in the RDP4 program. It covers NIb and CP coding regions. The putative parents are KP164988 (major parent, Argentina) and AB369278 (minor parent, South Korea). Both putative parents belong to the G3 molecular group. The phylogenetic analysis of the complete genome revealed that this isolate belongs to sub-group EM1 and clustered together with KP164988 sharing 98% and 94% nucleotide and amino acid identity, respectively. Therefore, leaf samples were collected from six different locations in the western part of the Czech Republic to check the occurrence of this emerging group revealed that the tested samples belonged to the same group, G3, based on the NIb-CP region (273 bp), and to sub-group EM1 based on the CP gene. This result provides evidence for the higher incidence of WMV in cucurbits compared to previous years in the Czech Republic (unpublished data).

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů