An improved method to efficiently acquire rice black-streaked dwarf virus viruliferous small brown planthoppers

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F23%3A10176162" target="_blank" >RIV/00027006:_____/23:10176162 - isvavai.cz</a>

Result on the web

<a href="https://www.frontiersin.org/articles/10.3389/fgene.2023.1111030/pdf" target="_blank" >https://www.frontiersin.org/articles/10.3389/fgene.2023.1111030/pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fgene.2023.1111030" target="_blank" >10.3389/fgene.2023.1111030</a>

Result language

angličtina

Original language name

An improved method to efficiently acquire rice black-streaked dwarf virus viruliferous small brown planthoppers

Original language description

Accurate phenotypic identification is the basis of research for resistance genetics and rice breeding for resistance to RBSDV disease. Obtaining rice black-streaked dwarf virus (RBSDV) viruliferous small brown planthoppers (SBPHs) with high transmission efficiency is an essential part of accurate phenotypic identification. Here, through quantifying number of RBSDV copies in infected rice plants, optimizing times of SBPHs fed on RBSDV-infected rice plants and leaf stage of rice seedlings, a method to acquire an RBSDV-carrying SBPH population more efficiently was improved. The results showed that rate of viruliferous SBPHs was significantly higher when fed on RBSDV-infected rice plants that had the copy numbers of RBSDV S10 of 3.0*10(4) and 1.1*10(4) than 8.3*10(2). Therefore, it is more efficient for SBPHs to acquire the virus when fed on RBSDV-infected rice plants that have copy numbers of RBSDV S10 above 1.1*10(4). The rate of viruliferous SBPHs were 50% and 54%, respectively, after the insects fed on RBSDV-infected rice plants for 7 and 9 days and being transferred to healthy rice seedlings for 5 and 3 days, which was significantly higher than those at other feeding times. The optimal inoculation leaf stage of rice seedlings was the 2-3-leaf stage (3 effective SBPHs per seedling for 72 h), but a high rate of viruliferous SBPHs may be suggested for inoculation of older rice seedlings.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in Genetics

ISSN

1664-8021

e-ISSN

—

Volume of the periodical

14

Issue of the periodical within the volume

FEB 1 2023

Country of publishing house

CH - SWITZERLAND

Number of pages

6

Pages from-to

1111030

UT code for WoS article

000935548200001

EID of the result in the Scopus database

2-s2.0-85148369480