Assessment of the Cadmium and Copper Phytoremediation Potential of the Lobularia maritima Thioredoxin 2 Gene Using Genetically Engineered Tobacco
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F23%3A10176203" target="_blank" >RIV/00027006:_____/23:10176203 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15640/23:73620300
Result on the web
<a href="https://www.mdpi.com/2073-4395/13/2/399/pdf?version=1675766206" target="_blank" >https://www.mdpi.com/2073-4395/13/2/399/pdf?version=1675766206</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/agronomy13020399" target="_blank" >10.3390/agronomy13020399</a>
Alternative languages
Result language
angličtina
Original language name
Assessment of the Cadmium and Copper Phytoremediation Potential of the Lobularia maritima Thioredoxin 2 Gene Using Genetically Engineered Tobacco
Original language description
Rapid global modernization, urbanization, and industrialization have accelerated the release of heavy metals, causing soil pollution. These highly noxious environmental pollutants induce oxidative stress in plants via stimulation of the production of reactive oxygen species (ROS). Thioredoxin (Trxs) is a highly conserved disulfide reductase that plays a crucial role in intracellular redox homeostasis in both eukaryotes and prokaryotes. Herein, the presence of heavy metals highly upregulated LmTrxh2 transcription in Lobularia maritima seedlings and its overexpression-conferred tolerance to Cd, Cu, Mn, and Zn in Saccharomyces cerevisiae. In addition, LmTrxh2-overexpressing tobacco plants had higher seedling survival rates than non-transgenic plants (NT), with enhanced root length and biomass production and reduced ROS accumulation, following Cd and Cu stress. These plants also accumulated more Cd, Cu, and Mn than the NT plants. Moreover, LmTrxh2 overexpression stimulated the transcription of genes encoding metallothioneins (Met-1, Met-2, Met-3, and Met-4), a copper transport protein, a Snakin/GASA protein (Snakin-2), and ROS-scavenging enzymes (SOD, APX1, and CAT), which might contribute to heavy metal tolerance in tobacco plants. These results suggest that LmTrxh2 overexpression helps to improve heavy metal tolerance by stimulating antioxidant capacities and the expression of several stress-responsive genes in plants.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)
Result continuities
Project
<a href="/en/project/EF16_019%2F0000827" target="_blank" >EF16_019/0000827: Plants as a tool for sustainable global development</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Agronomy-Basel
ISSN
2073-4395
e-ISSN
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Volume of the periodical
13
Issue of the periodical within the volume
2
Country of publishing house
CH - SWITZERLAND
Number of pages
14
Pages from-to
399
UT code for WoS article
000938129600001
EID of the result in the Scopus database
2-s2.0-85149124177