Predicting Blomia tropicalis allergens using a multiomics approach
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F23%3A10176765" target="_blank" >RIV/00027006:_____/23:10176765 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/23:10471256
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/epdf/10.1002/clt2.12302" target="_blank" >https://onlinelibrary.wiley.com/doi/epdf/10.1002/clt2.12302</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/clt2.12302" target="_blank" >10.1002/clt2.12302</a>
Alternative languages
Result language
angličtina
Original language name
Predicting Blomia tropicalis allergens using a multiomics approach
Original language description
The domestic mite Blomia tropicalis is a major source of allergens in tropical and subtropical regions. Despite its great medical importance, the allergome of this mite has not been sufficiently studied. Only 14 allergen groups have been identified in B. tropicalis thus far, even though early radioimmunoelectrophoresis techniques (27 uncharacterized allergen complexes) and comparative data based on 40 allergen groups officially recognized by the World Health Organization (WHO)/IUIS in domestic astigmatid mites suggest the presence of a large set of additional allergens.Here, we employ a multiomics approach to assess the allergome of B. tropicalis using genomic and transcriptomic sequence data and perform highly sensitive protein abundance quantification.Among the 14 known allergen groups, we confirmed 13 (one WHO/IUIS allergen, Blo t 19, was not found) and identified 16 potentially novel allergens based on sequence similarity. These data indicate that B. tropicalis shares 27 known/deduced allergen groups with pyroglyphid house dust mites (genus Dermatophagoides). Among these groups, five allergen-encoding genes are highly expressed at the transcript level: Blo t 1, Blo t 5, Blo t 21 (known), Blo t 15, and Blo t 18 (predicted). However, at the protein level, a different set of most abundant allergens was found: Blo t 2, 10, 11, 20 and 21 (mite bodies) or Blo t 3, 4, 6 and predicted Blo t 13, 14 and 36 (mite feces). We report the use of an integrated omics method to identify and predict an array of mite allergens and advanced, label-free proteomics to determine allergen protein abundance. Our research identifies a large set of novel putative allergens and shows that the expression levels of allergen-encoding genes may not be strictly correlated with the actual allergenic protein abundance in mite bodies.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)
Result continuities
Project
<a href="/en/project/LUAUS23082" target="_blank" >LUAUS23082: Comparative genomics and transcriptomics of allergens and putative allergen genes from stored product and house dust mites</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Clinical and Translational Allergy
ISSN
2045-7022
e-ISSN
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Volume of the periodical
13
Issue of the periodical within the volume
10
Country of publishing house
GB - UNITED KINGDOM
Number of pages
17
Pages from-to
e12302
UT code for WoS article
001173323400001
EID of the result in the Scopus database
2-s2.0-85173740002