Detection of Fusarium Head Blight Resistance Polymorphisms in Selected Barley Varieties by Single Base Primer Extension (SBE)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F23%3A10176769" target="_blank" >RIV/00027006:_____/23:10176769 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Detection of Fusarium Head Blight Resistance Polymorphisms in Selected Barley Varieties by Single Base Primer Extension (SBE)

Original language description

Fusarium Head Blight (FHB), a persistent threat caused by Fusarium fungi, leads to substantial annual losses in cereal yield and quality, particularly in barley. Understanding the complex genetic basis of FHB resistance in barley remains a significant challenge. Recent research has revealed that resistance to FHB, as well as the rate of accumulation of mycotoxins produced by the pathogen, is influenced by small-effect genes, modulated by plant morphology and environmental factors. These genes contain single nucleotide polymorphisms (SNPs) impacting plant phenotype to various extents. In other words, the resulting phenotype is determined, among other things, by polymorphisms located at multiple positions within the plant genome. Leveraging the genome wide association mapping (GWAS) method, we localized and identified putative genetic segments. Based on the known position of the polymorphisms in the genome, we developed 15 primers targeting significant SNPs. The Single Base Primer Extension (SBE) method facilitated the identification of specific nucleotides at the SNP site. Organizing these primers into two sets enabled multiplexing of reactions, thus significantly enhancing the overall efficiency of our workflow.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů