The Cryopreservation of Dormant Buds of Raspberry (Rubus idaeus L.)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F24%3A10177740" target="_blank" >RIV/00027006:_____/24:10177740 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
The Cryopreservation of Dormant Buds of Raspberry (Rubus idaeus L.)
Original language description
Cryoconservation enables long-term preservation of genetic resources in vegetatively propagated plants. For frost-resistant woody plants, cryobanking dormant buds is a cost-effective option, allowing regeneration by grafting. This study aimed to develop a cryopreservation method for dormant raspberry buds (Rubus idaeus L.) using pre-dehydration and two-step freezing, suitable for in vitro regeneration. Dormant raspberry shoots (varieties Sanibelle and Willamette) were collected midwinter, and uninodal segments were dehydrated in a freezer. Optimal dehydration levels were determined by studying low-temperature phase transitions, water state, and water content. Buds were rehydrated in wet peat and regrown in perlite. Results showed that dehydration reduced melting and crystallization temperatures, with water activity below 0.9 preventing crystallization during cooling. This critical dehydration level enabled up to 74% viable buds after cryopreservation.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)
Result continuities
Project
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Continuities
R - Projekt Ramcoveho programu EK
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů