What Happens Inside the Germinating Grain After Microbial Decontamination by Pulsed Electric Field? Data-Driven Multi-Omics Helps Find the Answer
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F25%3A10177942" target="_blank" >RIV/00027006:_____/25:10177942 - isvavai.cz</a>
Alternative codes found
RIV/60461373:22330/25:43931814
Result on the web
<a href="https://www.mdpi.com/1420-3049/30/4/924/pdf?version=1739785415" target="_blank" >https://www.mdpi.com/1420-3049/30/4/924/pdf?version=1739785415</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/molecules30040924" target="_blank" >10.3390/molecules30040924</a>
Alternative languages
Result language
angličtina
Original language name
What Happens Inside the Germinating Grain After Microbial Decontamination by Pulsed Electric Field? Data-Driven Multi-Omics Helps Find the Answer
Original language description
Pulsed electric field (PEF) has previously been recognized as a method of gentle food processing, and its use has been shown to be helpful in reducing the levels of toxigenic Fusarium micromycetes developed during malting. The aim of this study was to describe the effects of PEF on gene expression and metabolite production at the pre-finishing stage of barley malting by using a novel multi-omics data-driven approach. The study helps to uncover the processes occurring in the germinated grain and discusses the up-/downregulation of genes and metabolites in relation to fungal infection and/or PEF-induced abiotic stress. Among the factors upregulated by PEF and previously described as supportive against Fusarium diseases, we identified the increased expression of genes encoding vegetative gp1-like protein, which positively correlated with flavonoids, (methylsulfanyl)prop-2-enoates, triterpenoid glycosides, and indole alkaloids. On the other hand, some genes associated with barley resistance to fungal infection were also overexpressed in the untreated control (in particular, genes encoding ethylene response factor 3-like, putrescine hydroxycinnamoyltransferase 3-like, and dirigent protein 21-like). This study provides the first 'data-driven' basic research results that contribute to the understanding of the role of PEF as an effective fungal decontamination strategy and allows the formulation of new hypotheses related to Fusarium pathogen crosstalk.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
MOLECULES
ISSN
1420-3049
e-ISSN
1420-3049
Volume of the periodical
30
Issue of the periodical within the volume
4
Country of publishing house
CH - SWITZERLAND
Number of pages
15
Pages from-to
924
UT code for WoS article
001430091300001
EID of the result in the Scopus database
2-s2.0-85219174635