Pulsed Electric Field Treatment Modulates Gene Expression and Stress Responses in Fusarium-Infected Malting Barley
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F25%3A10178058" target="_blank" >RIV/00027006:_____/25:10178058 - isvavai.cz</a>
Alternative codes found
RIV/60461373:22330/25:43933159
Result on the web
<a href="https://www.mdpi.com/2223-7747/14/5/668/pdf?version=1740274520" target="_blank" >https://www.mdpi.com/2223-7747/14/5/668/pdf?version=1740274520</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/plants14050668" target="_blank" >10.3390/plants14050668</a>
Alternative languages
Result language
angličtina
Original language name
Pulsed Electric Field Treatment Modulates Gene Expression and Stress Responses in Fusarium-Infected Malting Barley
Original language description
Malting is a critical step in barley (Hordeum vulgare) processing, transforming grain into a key raw material for brewing and food production. However, the process is often compromised by Fusarium spp., pathogens responsible for Fusarium Head Blight, which reduces grain quality and safety. Pulsed electric field (PEF) treatment, a promising non-thermal technology, has been studied for its potential to inactivate microbial pathogens and mitigate infection-related stress. In this study, we investigated transcriptional responses in barley infected with Fusarium spp. during malting, both with and without PEF treatment. RNA sequencing identified over 12,000 differentially expressed genes (DEGs) across four malting stages, with the third stage (24 h of germination) showing the highest transcriptional activity. DEGs were significantly enriched in pathways related to oxidative stress management and abscisic acid signaling, underscoring their importance in stress adaptation. Barley treated with PEF exhibited fewer DEGs in later malting stages compared to untreated samples, suggesting that PEF alleviates stress induced by both Fusarium infection and the malting process. Enrichment analysis further revealed that PEF treatment up-regulated stress-related pathways while down-regulating genes associated with photosynthesis and cell wall biogenesis. These findings provide novel insights into barley stress responses during malting and highlight the potential of PEF as a tool for enhancing malt quality under stress conditions.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)
Result continuities
Project
<a href="/en/project/GA20-14649S" target="_blank" >GA20-14649S: Pulsed electric field as an innovative tool for decreasing Fusarium micromycetes and mycotoxins in the barley-malt-beer production chain</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plants-Basel
ISSN
2223-7747
e-ISSN
2223-7747
Volume of the periodical
14
Issue of the periodical within the volume
5
Country of publishing house
CH - SWITZERLAND
Number of pages
19
Pages from-to
668
UT code for WoS article
001442328800001
EID of the result in the Scopus database
2-s2.0-86000654646