Effect of Explant Physiology and Media Composition on Callogenesis of Vitellaria paradoxa Leaf Explants

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F25%3A10179172" target="_blank" >RIV/00027006:_____/25:10179172 - isvavai.cz</a>

Result on the web

<a href="https://www.mdpi.com/2311-7524/11/9/1127/pdf?version=1758505536" target="_blank" >https://www.mdpi.com/2311-7524/11/9/1127/pdf?version=1758505536</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/horticulturae11091127" target="_blank" >10.3390/horticulturae11091127</a>

Result language

angličtina

Original language name

Effect of Explant Physiology and Media Composition on Callogenesis of Vitellaria paradoxa Leaf Explants

Original language description

Vitellaria paradoxa (shea tree) is an economically and medicinally important species indigenous to sub-Saharan Africa. Although the species holds substantial value, domestication efforts have been constrained, primarily due to the absence of efficient propagation alternatives, especially for the East African subspecies (V. paradoxa subsp. nilotica) which remains understudied in tissue culture research. This study investigated the influence of leaf explant developmental stage and media composition on callogenesis and embryogenic potential in V. paradoxa subsp. nilotica. Thus, leaf explants from six distinct growth stages were cultured on Murashige and Skoog (MS) media supplemented with various concentrations of 2,4-D, TDZ, NAA, and BAP. Callogenesis was significantly influenced by explant age, media strength, and specific PGR combinations. Results revealed that explants from Stage III (11-15 days) and Stage IV (16-20 days) exhibited the highest callus induction rates (up to 100%), particularly on half-strength MS media containing 2.0 mg/L 2,4-D and 0.5-1.0 mg/L TDZ. Histological analysis suggests that varying responses at the different stages relate to chloroplast distribution, trichome density/orientation, and vascular tissue maturity. Pro-embryogenic structures were successfully induced, representing a developmental milestone with strong prospects for advanced stages of differentiation. The findings also emphasize the importance of explant physiology and media formulation in developing regeneration protocols for V. paradoxa from leaf explants.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Horticulturae

ISSN

2311-7524

e-ISSN

2311-7524

Volume of the periodical

11

Issue of the periodical within the volume

9

Country of publishing house

CH - SWITZERLAND

Number of pages

13

Pages from-to

1127

UT code for WoS article

001580026900001

EID of the result in the Scopus database

2-s2.0-105017396132