In vitro propagation of Tilia platyphyllos by axillary shoot proliferation and somatic embryogenesis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41410%2F03%3A00006026" target="_blank" >RIV/60460709:41410/03:00006026 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
In vitro propagation of Tilia platyphyllos by axillary shoot proliferation and somatic embryogenesis
Original language description
In vitro propagation of Tilia platyphyllos Scop. has been achieved by axillary shoot proliferation and somatic embryogenesis. The influence of tree age, explant source, genotype, and phytohormones on micropropagation of juvenile and mature trees of Tiliaplatyphyllos has been investigated. Nodal segments and shoot tips were used as initial explants for axillary shoot proliferation. Low concentration of cytokinin (BA, BPA, TDZ) plus auxin (IBA) stimulated fast shoot multiplication. Microshoots excised f rom proliferating cultures were rooted on low salt medium and produced trees were planted in the field. Embryogenic tissues were initiated from zygotic embryos cultured on MS medium suplemented with 2,4-D. After transfer of embryogenic tissues with developing embryoids on media lacking 2,4-D and supplemented with low concetration of IBA, the development of somatic embryos was enhanced. Secondary somatic embryogenesis led to the formation of new adventive somatic embryos. Trees produced fr
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GK - Forestry
OECD FORD branch
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Publication year
2003
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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