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In vitro propagation of Tilia platyphyllos by axillary shoot proliferation and somatic embryogenesis

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41410%2F03%3A00006026" target="_blank" >RIV/60460709:41410/03:00006026 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    In vitro propagation of Tilia platyphyllos by axillary shoot proliferation and somatic embryogenesis

  • Original language description

    In vitro propagation of Tilia platyphyllos Scop. has been achieved by axillary shoot proliferation and somatic embryogenesis. The influence of tree age, explant source, genotype, and phytohormones on micropropagation of juvenile and mature trees of Tiliaplatyphyllos has been investigated. Nodal segments and shoot tips were used as initial explants for axillary shoot proliferation. Low concentration of cytokinin (BA, BPA, TDZ) plus auxin (IBA) stimulated fast shoot multiplication. Microshoots excised f rom proliferating cultures were rooted on low salt medium and produced trees were planted in the field. Embryogenic tissues were initiated from zygotic embryos cultured on MS medium suplemented with 2,4-D. After transfer of embryogenic tissues with developing embryoids on media lacking 2,4-D and supplemented with low concetration of IBA, the development of somatic embryos was enhanced. Secondary somatic embryogenesis led to the formation of new adventive somatic embryos. Trees produced fr

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    GK - Forestry

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2003

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of Forest Science

  • ISSN

    1212-4834

  • e-ISSN

  • Volume of the periodical

    49

  • Issue of the periodical within the volume

    12

  • Country of publishing house

    CZ - CZECH REPUBLIC

  • Number of pages

    7

  • Pages from-to

    537-543

  • UT code for WoS article

  • EID of the result in the Scopus database